响应面法优化耙耙柑皮果胶超声辅助提取工艺及其抗氧化活性研究  

Optimization in Ultrasonic-Assisted Extraction Process of Pectin from Papagan Peels by Response Surface Methodology and Its Antioxidant Activity

作　　者：叶群丽[1] 冯可 梁大伟[1] 何靖柳[1] 章斌[1] YE Qunli;FENG Ke;LIANG Dawei;HE Jingliu;ZHANG Bin(Department of Pharmacy and Medical Laboratory,Ya′an Polytechnic College,Ya′an 625000,China)

机构地区：[1]雅安职业技术学院药学与检验学院,四川雅安625000

出　　处：《化学与生物工程》2022年第9期35-40,共6页Chemistry & Bioengineering

基　　金：雅安市重点科技计划项目(2020yyjskf12);四川省大学生创新创业训练计划项目(202113049616);雅安职业技术学院药物化学研究室建设项目(YZYJG201904),雅安职业技术学院自然科学科研项目(YZY2018ZKB01)。

摘　　要：以耙耙柑皮为原料,采用超声协同水浴振荡辅助酸法提取耙耙柑皮果胶,采用单因素实验考察了超声时间、超声温度、液料比、pH值、振荡时间及振荡温度等对果胶提取率的影响,采用响应面法进一步优化提取工艺,并研究了耙耙柑皮果胶的抗氧化活性。确定最佳提取工艺条件为:超声时间20 min、超声温度60℃、液料比40∶1(mL∶g)、pH值2.0、振荡时间20 min、振荡温度90℃,在此条件下,果胶提取率达28.42%。抗氧化实验结果表明,耙耙柑皮果胶对DPPH自由基、·O_(2)^(-)和·OH等3种自由基均具有清除能力,当果胶浓度为2.0 mg·mL^(-1)时,对3种自由基的清除率分别为43.9%、53.6%和35.0%。Taking Papagan peels as raw materials, we extracted pectin from Papagan peels by ultrasonic and water bath oscillation assisted acid extraction method.Moreover, we investigated the effects of ultrasonic time, ultrasonic temperature, liquid-solid ratio, pH value, oscillating time, and oscillating temperature on the extraction rate of pectin by single-factor experiments, and optimized the extraction conditions by response surface methodologies.Furthermore, we studied the antioxidant activity of pectin from Papagan peels.The optimal extraction conditions can be determined as follows: the ultrasonic time of 20 min, the ultrasonic temperature of 60 ℃,the liquid-solid ratio of 40∶1(mL∶g),the pH value of 2.0,the oscillating time of 20 min, and the oscillating temperature of 90 ℃.Under above conditions, the extraction rate of pectin from Papagan peels can reach 28.42%.In vitro antioxidant experiments show that the pectin from Papagan peels has scavenging abilities against DPPH free radicals, superoxide anion free radicals, and hydroxyl free radicals.When the concentration of pectin is 2.0 mg·mL^(-1),the scavenging rates of three free radicals are 43.9%,53.6%,and 35.0%,respectively.

关 键 词：耙耙柑皮 果胶 响应面法 超声辅助提取 水浴振荡 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]