动物双歧杆菌乳亚种XLTG11对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的缓解作用  

Alleviating Effect of Bifidobacterium animalis subsp.lactis XLTG11 on Ulcerative Colitis Induced by Sodium Dextran Sulfate in Mice

作　　者：马岩 王中江[2] 杨靖瑜 李哲 彭霞 陈昱凤 李柏良 MA Yan;WANG Zhongjiang;YANG Jingyu;LI Zhe;PENG Xia;CHEN Yufeng;LI Bailiang(Experimental Teaching Center,Shenyang Normal University,Shenyang 110034,China;College of Food Science,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]沈阳师范大学实验教学中心,辽宁沈阳110034 [2]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2022年第17期164-173,共10页Food Science

基　　金：辽宁省科技厅重点研发计划项目(2020JH2/10200039);沈阳市科技特派团项目(20-207-3-46)。

摘　　要：目的:通过葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导小鼠溃疡性结肠炎模型,探究动物双歧杆菌乳亚种XLTG11对小鼠溃疡性结肠炎的缓解作用。方法:将45只8周龄C57BL/6N雄鼠随机分为3组:正常组、模型组和双歧杆菌组,小鼠自由摄入3%(质量分数)DSS水溶液,建立溃疡性结肠炎模型,双歧杆菌组灌胃动物双歧杆菌乳亚种XLTG11(1×10^(7) CFU/d),测定小鼠体质量变化率、结肠长度、疾病活动指数(diseaseactivity index,DAI)和肠髓过氧化物酶(myeloperoxidase,MPO)活力,观察结肠组织病理学变化,测定结肠肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(interleukin,IL)-6、IL-1β、IL-10质量浓度,测定肠道菌群组成及短链脂肪酸含量,分析肠道屏障和核转录因子(nuclear transcription factor,NF)-κB信号通路相关基因的相对表达水平。结果:与模型组相比,动物双歧杆菌乳亚种XLTG11能够显著提高DSS诱导溃疡性结肠炎小鼠的体质量变化率(P<0.05)、结肠长度(P<0.01),极显著降低DAI、MPO活力和促炎细胞因子质量浓度(P<0.01),降低致病菌相对丰度和提高产短链脂肪防酸肠道菌群相对丰度,导致短链脂肪酸含量极显著增加(P<0.01),显著提高肠道屏障相关基因的表达(P<0.01),抑制核转录因子NF-κB信号通路的激活。结论:动物双歧杆菌乳亚种XLTG11可以提高DSS诱导溃疡性结肠炎小鼠的体质量变化率、结肠长度,降低DAI、MPO活力和促炎细胞因子水平,调节肠道菌群组成,提高短链脂肪酸含量,提高肠道屏障相关基因的表达并抑制NF-κB信号通路的激活,进而有效地缓解DSS诱导的小鼠溃疡性结肠炎。Objective:To investigate the relieving effect of Bifidobacterium animalis subsp.lactis XLTG11 on ulcerative colitis induced by dextran sulfate sodium(DSS)in mice.Methods:Forty-five eight-week-old C57BL/6N male mice were randomly divided into three groups:normal,model and bifidobacterium treatment.To induce ulcerative colitis,the mice were allowed free access to 3%DSS in water.B.animalis subsp.lactis XLTG11 was gavaged to the animals at a dose of 1×10^(7) CFU/d.The percentage change of body mass,colon length,disease activity index(DAI)and intestinal myeloperoxidase(MPO)activity were measured.Hstopathological changes were observed in the colon.Tumor necrosis factor(TNF)-α,interleukin 6(IL-6),IL-1β,and IL-10 contents in the colon tissue were determined.The gut microbiota composition and the contents of fecal short-chain fatty acids were evaluated.The relative expression levels of genes related to intestinal barrier and the nuclear transcription factor(NF)-κB signaling pathway were detected.Results:Compared to the model group,B.animalis subsp.lactis XLTG11 significantly increased the percentage change of body mass(P<0.05)and colon length(P<0.01),and decreased DAI,MPO activity and proinflammatory cytokine levels(P<0.01)in mice with DSS-induced ulcerative colitis.Moreover,it decreased the relative abundance of pathogenic bacteria,increased the relative abundance of short-chain fat acid-producing bacteria in the gut,significantly increased the contents of short-chain fat acids and the expression of genes related to intestinal barrier function(P<0.01),and inhibited the activation of the NF-κB signaling pathway.Conclusion:B.animalis subsp.lactis XLTG11 could increase the percentage change of body mass and colon length,decrease DAI,MPO activity,and proinflammatory cytokine levels,regulate the gut microbiota composition,increase fecal short-chain fatty acid contents and the expression of intestinal barrier-related genes and inhibit the NF-κB signaling pathway,thereby effectively relieving DSS-induced ulcerative coliti

关 键 词：动物双歧杆菌乳亚种 溃疡性结肠炎 肠道菌群 短链脂肪酸 核转录因子信号通路 

分 类 号：TS201.3[轻工技术与工程—食品科学]