Pharmacological insights into Chukrasia velutina bark:Experimental and computer-aided approaches  

作　　者：Israt Jahan Shahenur Alam Sakib Najmul Alam Mohuya Majumder Sanjida Sharmin A.S.M.Ali Reza 

机构地区：[1]Department of Pharmacy,International Islamic University Chittagong,Chittagong,Bangladesh [2]Department of Pharmacy,East West University,Dhaka,Bangladesh

出　　处：《Animal Models and Experimental Medicine》2022年第4期377-388,共12页动物模型与实验医学（英文）

摘　　要：Background:Chukrasia velutina is an enthnomedicinally used plant reported to have significant medicinal values.The present study aimed to explore the pharmacological activities of bark methanol extract using in vitro,in vivo and in silico models.Methods:The study was designed to investigate the pharmacological effects of methanol extract of Chukrasia velutina bark(MECVB)through in vitro,in vivo and in silico assays.Analgesic activity was tested using formalin-i nduced nociception and acetic acid-i nduced writhing assays while the antipyretic effect was tested using yeast-i nduced hyperthermia in mice model.The antioxidant effect was tested using the DPPH and reducing power assay and the cytotoxic screening was tested using the brine shrimp lethality bioassay.In addition,in silico studies were conducted using computer aided methods.Results:In the acetic acid-i nduced writhing assay,the extract showed 28.36%and 56.16%inhibition of writhing for doses of 200 and 400 mg/kg,respectively.Moreover,a dose-dependent formalin-i nduced licking response was observed in both early and late phase.In yeast-i nduced pyrexia,the MECVB exhibited(p<0.05)antipyretic effect.The extract demonstrated an ICvalue of 78.86μg/ml compared with ascorbic acid(IC23.53μg/ml)in the DPPH scavenging assay.The compounds sitosterol,5,7-dimethoxycoumarin and scopoletin were seen be effective in molecular docking scores against COX-I(2OYE),COX-II(6COX)and human peroxiredoxin 5(1HD2).In ADME/T analysis,5,7-dimethoxycoumarin and scopoletin satisfied Lipinski’s rule of five and thus are potential drug candidates.Conclusion:The bark of Chukrasia velutina showed significant analgesic and antipyretic properties and is a potential source of natural anti-oxidative agents.

关 键 词：ANTI-NOCICEPTIVE antioxidant ANTIPYRETIC Chukrasia velutina CYTOTOXICITY 

分 类 号：S853.74[农业科学—临床兽医学]