正交试验优化川明参多糖超临界CO_(2)萃取工艺  

Orthogonal Test Optimization of Supercritical CO_(2)Extraction Technology of Chuanmingshen Polysaccharide

作　　者：魏瑞霖 朱晨 赖朋 曾智康 段树华 陈晟 WEI Ruilin;ZHU Chen;LAI Peng;ZENG Zhikang;DUAN Shuhua;CHEN Sheng(Xihua University of College of Food and Biological Engineering,Chengdu,Sichuan 611730)

机构地区：[1]西华大学食品与生物工程学院,成都611730

出　　处：《食品工业》2022年第8期6-11,共6页The Food Industry

基　　金：四川省科技计划项目-巴中恩阳区川明参规范化种植与产地商品化处理(2021ZHFP0135);四川省科技计划项目-秦巴山区川明参全产业链建设关键技术攻关(22ZDYF0803)。

摘　　要：研究了超临界CO_(2)萃取法萃取川明参多糖的最佳工艺。通过单因素试验及正交试验对萃取工艺进行优化设计,采用苯酚-硫酸比色法测定萃取后川明参多糖含量,考察萃取温度、萃取时间、萃取压力和夹带剂(乙醇)浓度、料液比对川明参多糖萃取率的影响。得出结论:影响川明参多糖萃取率的先后顺序为萃取温度>萃取压力>萃取时间,其中萃取温度对川明参粗多糖萃取率具有极显著影响(P<0.01),萃取压力对川明参粗多糖萃取率具有显著影响(P<0.05)。最佳萃取工艺:在夹带剂90%乙醇,料液比1∶3(g/mL),萃取温度50℃,萃取时间2 h,萃取压力25 MPa下萃取2次。在该工艺条件下,川明参多糖的平均萃取率为23.81%。To study the best technology of supercritical CO_(2)extraction method for extracting polysaccharides from Chuanmingshen,the extraction process was optimized by single factor experiment and orthogonal experiment.The polysaccharide content of Chuanmingshen after extraction was determined by phenol-sulfuric acid colorimetry,and the extraction temperature,extraction time,extraction pressure,concentration of entrainer(ethanol),and liquid were investigated.Compare the effect on the extraction rate of Chuanmingshen polysaccharide.The conclusion was that the order of influencing the extraction rate of Chuanmingshen ginseng polysaccharide was extraction temperature>extraction pressure>extraction time.The extraction temperature had a very significant effect on the extraction rate of Chuanmingshen ginseng crude polysaccharide(P<0.01),and the extraction pressure had a significant effect on the extraction rate of Chuanmingshen ginseng polysaccharide.The extraction rate of crude polysaccharides had a significant effect(P<0.05).The best extraction process was 90%ethanol with entrainer concentration,1∶3(g/mL)ratio of material to liquid,extraction temperature of 50℃,extraction time of 2 h,and extraction pressure of 25 MPa.Under this technological condition,the average extraction rate of polysaccharides from Chuanmingshen was 23.81%.

关 键 词：川明参 超临界CO_(2)萃取 多糖 夹带剂 正交试验 

分 类 号：TS201.2[轻工技术与工程—食品科学]