高效液相色谱测定饮料中亮蓝合成着色剂条件的优化  

Optimization of Synthetic Colorant Conditions for Determination of Brilliant Blue in Beverages by High-Performance Liquid Chromatography

作　　者：金慧慧 JIN Huihui(Wenzhou Longwan for Disease Control and Prevention,Wenzhou 325024)

机构地区：[1]温州市龙湾区疾病预防控制中心,温州325024

出　　处：《食品工业》2022年第8期87-89,共3页The Food Industry

摘　　要：介绍一种运用自制固相萃取聚酰胺小柱-高效液相色谱技术检测饮料中亮蓝合成着色剂含量的方法。通过对样品溶液pH、淋洗液pH、加热到60℃等前处理步骤、样品溶液净化及浓缩步骤的改变,亮蓝回收率得到提高,达99.0%,相对标准偏差为0.20%,检出限为0.009 mg/L。建立的方法具有快速、准确、灵敏的特点。A method for the determination of bright blue synthetic colorant in beverages by using a self-made solid-phase extraction polyamide column-High-performance liquid chromatography technique was described.The recovery of brilliant blue was improved by changing the pre-treatment steps such as pH of sample solution,pH of leaching solution,heating to 60℃,purification and concentration of sample solution.The recovery was up to 99.0%,and the relative standard deviation was 0.20%;The detection limit was 0.009 mg/L.The established method was rapid,accurate and sensitive.

关 键 词：固相萃取 高效液相 亮蓝 饮料 

分 类 号：TS27[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]