发酵法制备鸡胚蛋抗氧化肽及清除自由基的研究  

Study on preparing antioxidant peptides from chicken embryo by fermentation and its scavenging ability on free radicals

作　　者：谷洁洁 秦翠丽[1,3] 赵思琪 侯颖 牛明福[1,2] 宫强[1,2] GU Jiejie;QIN Cuili;ZHAO Siqi;HOU Ying;NIU Mingfu;GONG Qiang(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023;College of Food and Bioengineering,Henan University of Science and Technology,Key Laboratory of Microbial Resources Exploitation and Utilization,Luoyang 471023;Henan Engineering Research Center of Food Microbiology,Luoyang 471023;Dynamiker Biotechnology(Tianjin)Co.,Ltd.,Tianjin 300480)

机构地区：[1]河南科技大学食品与生物工程学院,洛阳471023 [2]河南科技大学微生物资源开发与利用校级重点实验室,洛阳471023 [3]食品微生物河南省工程技术研究中心,洛阳471023 [4]丹娜(天津)生物科技股份有限公司,天津300480

出　　处：《中国食品添加剂》2022年第9期113-119,共7页China Food Additives

基　　金：河南省重点攻关项目(No.172102310327)。

摘　　要：本文研究了一种能节约时间、降低成本且清除自由基能力强的抗氧化肽制备方法。该方法是以鸡胚蛋为原料,利用枯草芽孢杆菌发酵制备鸡胚蛋抗氧化肽。试验用响应面法优化制备,以DPPH·清除率为指标,确定发酵制备工艺的最佳发酵时间、底物浓度和转速,并验证此条件下制备的鸡胚蛋抗氧化肽对自由基的清除能力。结果表明,最佳发酵工艺为:发酵时间为55h,底物浓度为67g/L,转速为193r/min,在此条件下制备的鸡胚蛋抗氧化肽具有较好的清除自由基能力。它对DPPH·清除率高达53.04%,对DPPH·、OH·、O^(2-)·以及ABTS^(+)·的EC_(50)分别为0.94mg/mL、0.26mg/mL、0.22mg/mL、0.012mg/mL。In this paper,an antioxidant peptide preparation which can save time,reduce cost and have strong free radical scavenging ability was studied. The method was used to prepare antioxidant peptides from chicken embryo by bacillus subtilis fermentation. Response surface methodology was used to optimize the preparation. DPPH · clearance rate was taken as the index to determine the optimal fermentation time,substrate concentration and rotation speed as well as the free radical scavenging ability of chicken embryo antioxidant peptide. The results showed that the optimal fermentation conditions were :fermentation time 55h,substrate concentration 67g/L,rotation speed 193r/min. Under these conditions,the prepared chicken embryo antioxidant peptide had better scavenging ability of free radicals. The scavenging rate of DPPH·was 53.04%,and the EC_(50) of DPPH·,OH·,O^(2-)·and ABTS^(+)·were 0.94mg/mL,0.26mg/mL,0.22mg/mL and 0.012mg/mL,respectively.

关 键 词：鸡胚蛋 发酵 鸡胚蛋抗氧化肽 响应面 自由基 

分 类 号：O629.72[理学—有机化学] Q815[理学—化学]