牡丹江2株香蘑属菌株的鉴定及驯化研究  

Identification and Domestication of Two Strains of Lepista spp. in Mudanjiang

作　　者：盛春鸽[1] 王延锋[1] 史磊[1] 潘春磊[1] 王金贺[1] 刘姿彤[1] 张鹏[1] 于海洋[1] 赵静 SHENG Chun-ge;WANG Yan-feng;SHI Lei;PAN Chun-lei;WANG Jin-he;LIU Zi-tong;ZHANG Peng;YU Hai-yang;ZHAO Jing(Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157000,China)

机构地区：[1]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157000

出　　处：《中国食用菌》2022年第9期26-29,33,共5页Edible Fungi of China

基　　金：农业基础性长期性科技工作观测监测“国家农用微生物伊春观测实验站”(NAES060AM03);2021年黑龙江省农业科学院“创新跨越工程”中试项目(2021ZSXM012);黑龙江省农业科学院2019年度院级科研项目(2019KYJL011)。

摘　　要：通过表型鉴定和ITS测序相结合的方法鉴定香蘑属2个菌株。结果表明,2个供试菌株1株是花脸香蘑,另1株是紫丁香蘑。对香蘑属菌株进行驯化研究发现,花脸香蘑菌丝体的最适生长温度为24℃,子实体的最适宜发育温度为22℃~26℃,利用玉米秸秆发酵料栽培,成功驯化花脸香蘑菌株。紫丁香蘑菌丝体的最适生长温度为20℃,没有获得子实体。Two strains of Lepista spp. were identified by phenotypic identification combined with ITS sequence. The results showed that, one strain was Lepista sordida, and the other was Lepista nuda. The domestication of Lepista spp. strains showed that the optimum temperature for L. sordida mycelial growth was 24 ℃, 22 ℃-26 ℃ was the optimum temperature for development of fruit bodies. The strain of L. sordida was successfully domesticated by cultivating with corn stalk fermentation substrate. And the optimum temperature for L. nuda mycelial growth was 20 ℃, and no fruit bodies were obtained.

关 键 词：香蘑属 菌株鉴定 ITS 驯化栽培 

分 类 号：S646.1[农业科学—蔬菜学]