灵芝水溶性和碱溶性多糖的抗氧化性能比较研究  

Comparative Study on Antioxidant Activity of Water Soluble and Alkali Soluble Polysaccharides from Ganoderma lucidum

作　　者：韩伟 陈炼茹 郑丹婷 卜原玲 姚泽远 HAN Wei;CHEN Lianru;ZHENG Danting;BU Yuanling;YAO Zeyuan(Engineering Research Centre of the Ministry of Education for Pharmaceutical Engineering and Process Chemistry,Shanghai 200237,China;Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海200237 [2]华东理工大学药学院上海市新药设计重点实验室,上海200237

出　　处：《徐州工程学院学报（自然科学版）》2022年第3期33-40,共8页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：上海市科技兴农重点攻关项目[沪农科创字(2018)第1-1号]。

摘　　要：以沪农灵芝、龙芝2号子实体为原料,分别采用微波提取、碱提醇沉法得到灵芝水溶性和碱溶性多糖.水溶性多糖经体积分数30%、50%、80%乙醇分步沉淀,分别得到不同组分的多糖GLPa、GLPb和GLPc溶液;碱溶性多糖经DEAE-纤维素52柱和葡聚糖凝胶Sephadex G-100柱层析,得到纯化的碱溶性多糖GLPj.采用高效凝胶渗透色谱对不同多糖组分的均一性进行了鉴定,同时测定了相对分子质量.结果表明,低体积分数乙醇沉淀得到的水溶性多糖GLPa相对分子质量达百万级,高体积分数乙醇沉淀得到的水溶性多糖GLPb和GLPc相对分子质量为十万级,碱溶性多糖GLPj相对分子质量小于1万.体外抗氧化活性实验表明,各灵芝多糖均具有还原能力,对羟基自由基及DPPH自由基具有一定的清除作用,灵芝水溶性多糖普遍比碱溶性多糖表现出更好的抗氧化活性,其中GLPa的各项能力较其他多糖均为最佳,抗氧化活性可能与相对分子质量有关.The water-soluble polysaccharides and alkali-soluble polysaccharides of Ganoderma lucidum were obtained by water extraction,alkali extraction and alcohol precipitation from Ganoderma lucidum SH and Longzhi No.2.Water-soluble polysaccharides were precipitated by 30%,50%and 80%ethanol step by step to obtain different fractions of polysaccharides GLPa,GLPb and GLPc.The alkali-soluble polysaccharide GLPj was obtained by DEAE-cellulose 52 column and Sephadex G-100 column chromatography.The molecular weights of each purified polysaccharide component were determined by HPSEC.The results showed that the molecular weight of water-soluble polysaccharide obtained by low concentration ethanol precipitation(GLPa)was one million,water-soluble polysaccharide obtained by high concentration ethanol precipitation(GLPb and GLPc)was 1×105,and alkali-soluble polysaccharide(GLPj)was less than 1×104.In vitro antioxidant activity experiments showed that,all the polysaccharides of Ganoderma lucidum had reducing ability and had certain scavenging effect on hydroxyl radicals and DPPH radicals.Water-soluble polysaccharides generally showed better antioxidant activity than alkali-soluble polysaccharides,among which GLPa had the best ability compared with other polysaccharides,and this may be related to molecular weight.

关 键 词：灵芝 水溶性多糖 碱溶性多糖 相对分子质量 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]