常用食品添加剂脱氢乙酸钠的急性毒性效应研究  

The acute toxicity of sodium dehydroacetate as a common food additive

作　　者：杜宏举[1] 谭壮生[1] 张楠[1] 高珊[1] 敬海明[1,2] 张鹏[1] 李国君[1,2] DU Hong-ju;TAN Zhuang-sheng;ZHANG Nan;GAO Shan;JING Hai-ming;ZHANG Peng;LI Guo-jun(Beijing Center for Disease Prevention and Control,Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing 100013,China;School of Public Health,Capital Medical University,Beijing 100069,China)

机构地区：[1]北京市疾病预防控制中心,北京市食物中毒诊断溯源技术重点试验室,北京100013 [2]首都医科大学公共卫生学院,北京100069

出　　处：《毒理学杂志》2022年第4期292-296,303,共6页Journal of Toxicology

基　　金：国家重点研发计划(2018YFC1603102,2018YFC1602705)。

摘　　要：目的采用体内和体外试验,研究常用食品添加剂脱氢乙酸钠(DHA-S)的急性毒性效应。方法(1)昆明种小鼠单次经口灌胃4640、2150、1000和464 mg/kg·bw剂量的DHA-S,观察中毒症状和死亡情况,确定半数致死量(LD_(50))。(2)秀丽隐杆线虫(野生型N2)L4期幼虫,分别用0~100 mg/ml DHA-S染毒24 h,计数各剂量组线虫死亡率(%)。(3)体外培养的中国仓鼠肺细胞(CHL),分别用0~5.0 mg/ml DHA-S染毒24 h,计算各剂量组细胞相对增殖率(%)。结果(1)雌性、雄性小鼠急性经口LD_(50)分别为1470和2000 mg/kg·bw,均属低毒级。(2)线虫的LC_(50)为1.53 mg/ml时,具有毒性。(3)细胞LC_(50)为1.78 mg/ml,毒性分级为3级,属中等毒性。结论不同剂量DHA-S急性染毒可对试验动物、模式生物及哺乳动物细胞产生明显的毒性效应。Objective The acute toxic effects of sodium dehydroacetate(DHA-S),a common food additive,were studied in vivo and in vitro.Method(1)Kun-ming mice were given a single oral gavage of 4640,2150,1000,464 mg/kg·bw of DHA-S,and the poisoning symptoms and death were observed to determine the median lethal dose(LD_(50)).(2)The L4 larvae of Caenorhabditis elegans(wild type N2)were exposed to 0~100 mg/ml DHA-S for 24 hours respectively,and the mortality of nematodes in each dose group was counted(%).(3)Chinese hamster lung cells(CHL)cultured in vitro were exposed to 0~5.0 mg/ml DHA-S for 24 hours respectively,and the relative proliferation rate(%)of cells in each dose group was calculated.Results(1)The acute oral LD_(50) of female and male mice were 1470 mg/kg·bw and 2000 mg/kg·bw,respectively,which were of low toxicity.(2)The LC_(50) of nematode was 1.53 mg/ml,which was toxic.(3)The LC_(50) of CHL cells was 1.78 mg/ml,and the toxicity grade was grade 3,belonging to medium toxicity.Conclusion Acute exposure to different doses of DHA-S can produce significant toxic effects on experimental animals,model organisms and mammalian cells.

关 键 词：脱氢乙酸钠 急性毒性 食品添加剂 防腐剂 

分 类 号：R114[医药卫生—卫生毒理学] R155[医药卫生—公共卫生与预防医学] R99