Efficient reduction ofβ-lactoglobulin allergenicity in milk using Clostridium tyrobutyricum Z816  

作　　者：Qianru Zhao Yuwei Wang Zhengming Zhu Quanyu Zhao Liying Zhu Ling Jiang 

机构地区：[1]College of Biotechnology and Pharmaceutical Engineering,State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing Tech University,Nanjing 210009,China [2]College of Food Science and Light Industry,Nanjing Tech University,Nanjing 210009,China [3]School of Pharmaceutical Sciences,Nanjing Tech University,Nanjing 211816,China [4]School of Chemistry and Molecular Engineering,Nanjing Tech University,Nanjing 210009,China

出　　处：《Food Science and Human Wellness》2023年第3期809-816,共8页食品科学与人类健康（英文）

基　　金：supported by the National Key R&D Program of China(2017YFC1600404);the National Natural Science Foundation of China(31922070,22008114);the Natural Science Foundation of Jiangsu Province(BK20180038,BK20200684)。

摘　　要：Milk allergy is one of the most common food allergies,affecting 6%of young children,andβ-lactoglobulin(β-LG)is the main milk allergen.Clostridium tyrobutyricum Z816 was selected for the degradation ofβ-LG,which was successfully reduced by about 90%using permeabilized bacteria under the optimized conditions.The hydrolyzed peptides were identified by liquid chromatography-tandem mass spectrometry(LC-MS/MS)and analyzed by molecular modeling,which indicated that C.tyrobutyricum Z816 could effectively degrade the antigenic epitopes ofβ-LG.Finally,the concentration and digestibility ofβ-LG in actual samples was quantified using enzyme-linked immunosorbent assay(ELISA)and gastrointestinal digestion simulation experiments.The results showed more than 92%ofβ-LG in actual samples was hydrolyzed,and the gastric and total digestibility of whey protein isolate(WPI)was improved by 85.96%and 64.51%,respectively.Therefore,C.tyrobutyricum Z816 offers an effective method to degradeβ-LG and reduce the occurrence of milk allergies,which has great significance for the development of hypoallergenic dairy products.

关 键 词：Milk allergy Β-LACTOGLOBULIN Clostridium tyrobutyricum Permeabilized bacteria 

分 类 号：TS20[轻工技术与工程—食品科学]