The mechanisms of melanogenesis inhibition by glabridin:molecular docking,PKA/MITF and MAPK/MITF pathways  

作　　者：Chunxing Pan Xiaoying Liu Yating Zheng Zejun Zhang Yongliang Li Biao Che Guangrong Liu Lanyue Zhang Changzhi Dong Haji Akber Aisa Zhiyun Du Zhengqiang Yuan 

机构地区：[1]School of Biomedical and Pharmaceutical Sciences,Guangdong University of Technology,Guangzhou 510006,China [2]Infinitus(China)Company Ltd.,Guangzhou 510663,China [3]Foshan Allen Conney Biological Technology Co.,Ltd.,Foshan 528225,China [4]UniversitéParis Diderot,Sorbonne Paris Cité,ITODYS,UMR 7086 CNRS,France [5]The Key Laboratory of Plant Resources and Chemistry of Arid Zone,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Ürümqi 830011,China

出　　处：《Food Science and Human Wellness》2023年第1期212-222,共11页食品科学与人类健康（英文）

基　　金：supported by the Inner Mongolia Autonomous Region Science and Technology Revitalization Foundation (2021CG0029);the National Natural Science Foundation of China (22178070)

摘　　要：Glabridin is the main ingredient of hydrophobic fraction in licorice extract and has been shown to have anti-melanogenesis activity in skins.However,the underlying mechanism(s)remain not completely understood.The aim of this study is thus to elucidate the possible mechanisms related to the melanogenesis suppression by glabridin in cultured B16 murine melanoma cells and in UVA radiation induced hyperpigmentation model of BALB/c mice as well.Molecular docking simulations revealed that between catalytic core residues and the compound.The treatment by glabridin significantly downregulated both transcriptional and/or protein expression of melanogenesis-related factors including melanocyte stimulating hormone receptor(MC1R),microphthalmia-associated transcription factor(MITF),tyrosinase(TYR),TYR-related protein-1(TRP-1)and TRP-2 in B16 cells.Both PKA/MITF and MAPK/MITF signaling pathways were found to be involved in the suppression of melanogenesis by glabridin in B16 cells.Also in vivo glabridin therapy significantly reduced hyperpigmentation,epidermal thickening,roughness and inflammation induced by frequent UVA exposure in mice skins,thus beneficial for skin healthcare.These data further look insights into the molecular mechanisms of melanogenesis suppression by glabridin,rationalizing the application of the natural compound for skin healthcare.

关 键 词：GLABRIDIN MELANOGENESIS Molecular docking PKA/MITF pathway MAPK/MITF pathway 

分 类 号：R285.5[医药卫生—中药学]