响应面法优化糯玉米淀粉酶解工艺制备抗消化淀粉  

Optimization of Hydrolysis Technology for Preparation of Anti-digestible Starch from Waxy Corn Starch Using Response Surface Methodology

作　　者：孙琳琳 李克文[2] 孙桂莲 龚魁杰[1,2] 刘开昌 SUN Linlin;LI Kewen;SUN Guilian;GONG Kuijie;LIU Kaichang(Crop Research Institute,Shandong Academy of Agricultural Sciences,Ji'nan,Shandong 250100,China;Baolingbao Biotechnology Co.,Ltd.,Yucheng,Shandong 251200,China)

机构地区：[1]山东省农业科学院作物研究所,山东济南250100 [2]保龄宝生物股份有限公司,山东禹城251200

出　　处：《农产品加工》2022年第18期37-40,46,共5页Farm Products Processing

基　　金：山东省泰山产业领军人才工程专项经费资助项目(2019.11);山东省玉米产业技术体系项目(SDAIT-02-11);山东省枣庄英才集聚工程项目(2019.6);山东省重点研发计划(医用食品专项计划)项目(2019YYSP013)。

摘　　要：研究不同因素对糯玉米淀粉酶解获得慢活化化淀粉(SDS)和抗性淀粉(RS)含量的影响,采用异淀粉酶对糯玉米淀粉进行酶解,以SDS+RS含量为指标,通过单因素试验和响应面法对糯玉米淀粉酶解工艺进行优化。确定其最佳工艺参数为酶解时间49 min,酶添加量2.7 U/g,老化时间23.8 h,酶解温度49℃,在此条件下SDS+RS含量为34.103%。In this study,the effects of different factors on the contents of SDS and RS obtained by enzymatic hydrolysis of waxy corn starch were studied.Waxy corn starch was hydrolyzed by isoamylase.The hydrolysis technology of waxy corn starch was optimized by single factor test and response surface test with SDS+RS content as index.The optimum technological parameters were enzymatic hydrolysis time 49 min,enzyme dosage 2.7 U/g,aging time 23.8 h,and enzymatic hydrolysis temperature 49℃,under these conditions the SDS+RS content was 34.103%.

关 键 词：糯玉米淀粉 响应面 慢活化化淀粉(SDS) 抗性淀粉(RS) 

分 类 号：TS201.3[轻工技术与工程—食品科学]