毛冠鹿(Elaphodus cephalophus)体细胞的染色体研究  

SOMATIC CHROMOSOME STUDIES OF THE TUFTED DEER (ELAPHODUS CEPHALOPHUS)

作　　者：张锡然[1] 王建华[1] 陈玉泽[1] Zhang Xiran;Wang Jianhua;Chen Yuze(Kunming Institute of Zoology,Academia Sinica)

机构地区：[1]中国科学院昆明动物研究所

出　　处：《Zoological Research》1983年第1期89-93,共5页动物学研究（英文）

摘　　要：本文首次报道我国特有的珍贵动物毛冠鹿的染色体数目及特征,其二倍染色体众数雄性为2n=48,雌性为2n=47。雌雄两性的核型中均有几条难以鉴别的染色体,如雌性的第23对染色体,24号染色体;雄性的第23号,24号、25号、26号染色体,G-带分析表明,雄性的第25号,26号染色体正相当于雌性的24号染色体的长臂和短臂。C-带,G-带观察表明,雌性的第23对,24号染色体,雄性的23号,24号,25号染色体异染色质特别丰富,推测这几条染色体可能参与性别的决定,但正确的性决定机制及性染色体的鉴别有待进一步研究。Ag—As技术揭示,核仁组织者均位于第一对和第二对染色体的次缢痕处。此外,本文还测定了该种动物姐妹染色单体的交换频率(SCE),其平均值为每细胞6.2。The karyotype of the Tufted Deer (Elaphodus cephalophus) is first investigated with cultured fibrolasts from the lung and skin of two animals (one male and one female). The diploid number is 48, in the male and 47, in the female. G-band analysis shows that chromosomes 25 and 2G of the male just correspond to the long and short arms of chromosome 24 of the female, respectively. C-band observations shows that a pair of chromosome 23 and a chromosome 24 in the female and a chromosome 23, a chromosome 24 and a chromosome 25 in the male all contain rich heterochromatin. We think that these specific chromosomes probably relate to the sexual determination. The Ag-AS technique reveals clearly silver stained spots at the secondary constrictions of two pairs of autosomes 1 and 2. In addition, the spontaneous frequencies of the sister chromatid exchanges (SCE) of this species are calculated. The average SCE number er cell is 6.2.

关 键 词：姐妹染色单体 核仁组织者 短臂 染色体研究 异染色质 4号染色体 性染色体 次缢痕 

分 类 号：R73[医药卫生—肿瘤]