Generation ofαGal-enhanced bifunctional tumor vaccine  

作　　者：Jian He Yu Huo Zhikun Zhang Yiqun Luo Xiuli Liu Qiaoying Chen Pan Wu Wei Shi Tao Wu Chao Tang Huixue Wang Lan Li Xiyu Liu Yong Huang Yongxiang Zhao Lu Gan Bing Wang Liping Zhong 

机构地区：[1]National Center for International Research of Biotargeting Theranostics,Guangxi Key Laboratory of Biotargeting Theranostics,Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy,Guangxi Talent Highland of Biotargeting Theranostics,Guangxi Medical University,Nanning 530021,China [2]The First Affiliated Hospital,Guangxi University of Chinese Medicine,Nanning 530023,China [3]The First People’s Hospital of Changde City,Changde 415003,China [4]Department of Spine Surgery,the Second Xiangya Hospital of Central South University,Changsha 410011,China

出　　处：《Acta Pharmaceutica Sinica B》2022年第7期3177-3186,共10页药学学报（英文版）

基　　金：supported,in part,by grants from the State Project for Essential Drug Research and Development(No.2019ZX09301132,China);National Natural Science Foundation of China(Nos.82060562 and 82072340);the Scientific and Technological Innovation Major Base of Guangxi(No.2018-15Z04,China);Guangxi Key Research and Development Project(No.AB20117001,China);Guangxi Natural Science Foundation(No.2018JJA140524,China)。

摘　　要：Hepatocellular carcinoma(HCC)is a common malignant tumor with poor prognosis and high mortality.In this study,we demonstrated a novel vaccine targeting HCC and tumor neovascular endothelial cells by fusing recombinant MHCC97H cells expressing porcine a-1,3-galactose epitopes(a Gal)and endorphin extracellular domains(END)with dendritic cells(DCs)from healthy volunteers.END^(+)/Gal^(+)-MHCC97H/DC fusion cells induced cytotoxic T lymphocytes(CTLs)and secretion of interferon-gamma(IFN-γ).CTLs targeted cells expressing a Gal and END and tumor angiogenesis.The fused cell vaccine can effectively inhibit tumor growth and prolong the survival time of human hepatoma mice,indicating the high clinical potential of this new cell based vaccine.

关 键 词：aGal ENDOGLIN Dendritic cells Fusion cells Hepatocellular carcinoma Tumor neovascular endothelial cells Cytotoxic T lymphocytes IMMUNOTHERAPY 

分 类 号：R730.51[医药卫生—肿瘤]