Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R  

作　　者：Pengfei Zhu Lihui Ren Ying Zhu Jing Dai Huijie Liu Yuli Mao Yuandong Li Yuehui He Xiaoshan Zheng Rongze Chen Xiaoting Fu Lili Zhang Lijun Sun Yuanqi Zhu Yuetong Ji Bo Ma Yingchun Xu Jian Xu Qiwen Yang 

机构地区：[1]Single‐Cell Center,CAS Key Laboratory of Biofuels,Shandong Key Laboratory of Energy Genetics and Shandong Energy Institute,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao,China [2]University of Chinese Academy of Sciences,Beijing,China [3]College of Information Science&Engineering,Ocean University of China,Qingdao,China [4]Department of Clinical Laboratory,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing,China [5]Graduate School,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing,China [6]Department of Clinical Laboratory,Affiliated Hospital of Qingdao University,Qingdao University,Qingdao,China [7]Qingdao Single‐Cell Biotechnology,Co.,Ltd.,Qingdao,China [8]The Bioland Laboratory,Guangzhou,China

出　　处：《mLife》2022年第3期329-340,共12页微生物（英文）

基　　金：We thank Yang Liu for graphics support.This study was supported by CAS(XDB29050400,KFJ‐STS‐QYZX‐087);NSFC(31827801,82072318);National Key Research and Development Program of China(2018YFE0101800,2021YFC2301002);Traditional Chinese Medicine Science and Technology Development Program of Shandong Province(No.2019‐0596).

摘　　要：Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and unstable.Using the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman microspectroscopy.Featuring a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by>10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman spectra.The Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii isolates.Further tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based results.The automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials.

关 键 词：antimicrobial susceptibility test laboratory automation RAMAN SEPSIS TIGECYCLINE 

分 类 号：R96[医药卫生—药理学]