Exhaled volatile organic compounds for cholangiocarcinoma diagnosis  

作　　者：Nanicha Siriwong Thanikan Sukaram Rossarin Tansawat Terapap Apiparakoon Thodsawit Tiyarattanachai Sanparith Marukatat Rungsun Rerknimitr Roongruedee Chaiteerakij 

机构地区：[1]Department of Medicine,Faculty of Medicine,Chulalongkorn University,Bangkok,Thailand [2]Division of Gastroenterology,Department of Medicine,Faculty of Medicine,Chulalongkorn University,and King Chulalongkorn Memorial Hospital,Thai Red Cross Society,Bangkok,Thailand [3]Department of Food and Pharmaceutical Chemistry,Faculty of Pharmaceutical Sciences,Chulalongkorn University,Bangkok,Thailand [4]Department of Computer Engineering,Faculty of Engineering,Chulalongkorn University,Bangkok,Thailand [5]Clinical Sciences PhD International Program,Faculty of Medicine,Chulalongkorn University,Thailand [6]Image Processing and Understanding Team,Artificial Intelligence Research Group,National Electronics and Computer Technology Center(NECTEC),Thailand [7]Center of Excellence for Innovation and Endoscopy in Gastrointestinal Oncology,Chulalongkorn University,Bangkok,Thailand

出　　处：《Liver Research》2022年第3期191-197,共7页肝脏研究（英文）

基　　金：This work was funded by the Thailand Research Fund(TRF)and The Office of the Higher Education Commission(OHEC)(MRG6180227 to R.Chaiteerakij);Research Grant:GAT2018 to R.Chaiteerakij,The Gastroenterological Association of Thailand;and Research Grant for New Scholar Ratchadaphiseksomphot Endowment Fund Chulalongkorn University(RGN_2559_055_10_30 to R.Chaiteerakij).

摘　　要：Objectives:The difficulties in the early detection consequent to the lack of sensitive biomarkers render patients with cholangiocarcinoma(CCA)to have poor outcomes.Recently,sensitive and specific volatile organic compounds(VOCs)were identified in several cancers.However,the VOC profiles in CCA are not well-studied.Thus,we investigated the VOC profiles in exhaled breath of CCA patients and controls.Methods:We prospectively collected exhaled breath samples from 30 consecutive patients newly diagnosed with CCA and 30 controls who did not have CCA(seven had benign biliary strictures and 23 had other medical conditions).Exhaled VOCs were identified using gas chromatography mass spectrometry Triple Quadrupoles system.Analysis of the significant differences in VOCs between cases and controls was conducted using supervised multivariate regression analysis.Further validation was performed for these VOCs in another cohort of 18 CCA patients and 22 controls.Results:Levels of six compounds were significantly different between CCA patients and controls,namely,acetone,isopropyl alcohol,dimethyl sulfide,1,4-pentadiene,allyl methyl sulfide,and N,N-dimethylacetamide.Acetone and dimethyl sulfide were independently associated with CCA as demonstrated in the multivariate analysis.Using the cut-off value of 8.59107 arbitrary unit(AU),acetone had a sensitivity and specificity of 82.1%and 75.8%,respectively,with an area under the receiving operator curve(AUROC)of 0.85 for the CCA diagnosis.Acetone level was also significantly different between cases and controls in the validation cohort.Using the same cut-off value,the sensitivity,specificity,and AUROC was 59.1%,66.7%,and 0.85,respectively.Conclusion:Breath analysis may potentially be useful for CCA diagnosis.A cohort of patients with earlystage CCA in further studies is needed to confirm the ability of exhaled VOCs for the early detection of CCA.

关 键 词：Cholangiocarcinoma(CCA) Bile duct cancer Volatile organic compounds(VOCs) BIOMARKER Cancer screening Diagnostic model 

分 类 号：R735.8[医药卫生—肿瘤]