天然产物中抗微生物肽的高效筛选方法及研究进展  

A Review of Methods and Progress in Highly Efficient Screening of Antimicrobial Peptides from Natural Products

作　　者：杨鸿雁 单紫轩 赖亮 段俊 汪锦才 霍嘉茵 郭嘉亮 YANG Hongyan;SHAN Zixuan;LAI Liang;DUAN Jun;WANG Jincai;HUO Jiayin;GUO Jialiang(School of Medicine,Foshan University,Foshan 528000,China;Pharmacy College,Jinan University,Guangzhou 510632,China;Sinopharm Group Fengliaoxing(Foshan)Pharmaceutical Co.,Ltd.,Foshan 528000,China)

机构地区：[1]佛山科学技术学院医学院,广东佛山528000 [2]暨南大学药学院,广东广州510632 [3]佛山冯了性药业有限公司,广东佛山528000

出　　处：《食品工业科技》2022年第21期28-35,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(81872832,82173781);广东省基础与应用基础研究基金自然科学基金项目(2019A1515010806);广东省教育厅重点领域专项(2020ZDZX2057);佛山科学技术学院口腔医学科研培育项目。

摘　　要：面对耐药病原体的挑战,天然抗微生物肽(Antimicrobial peptide,AMP)成为了开发新一代抗菌候选药物的重要来源之一,在食品、农业等领域同样有着广泛的应用。然而,从天然产物中快速筛选获得抗微生物肽仍然存在着低效、高耗等种种困难与挑战。本文首先介绍了抗微生物肽的作用机制(包括膜作用机制与非膜作用机制);然后重点综述了AMP的高效筛选方法,包括整体细菌吸附结合法、细胞膜色谱法、磷脂膜色谱法、毛细管电泳法、比色法、薄层色谱法、荧光筛选法、高通量测序法和数据库挖掘法等;此外,展望了高效发掘抗微生物肽的发展方向。本论文为从纷繁复杂的天然产物体系中发现抗微生物肽提供了可参考的科学依据。Natural antimicrobial peptides(AMPs) are promising candidates for developing a generation of new antimicrobials to meet the challenge of antibiotic-resistant pathogens, and they are extensively applied in medicine, food,agriculture and other fields. However, the rapid screening of AMPs from natural products still has many difficulties and challenges, such as low efficiency and high consumption. This paper firstly introduces the action mechanism of AMPs(including membrane action mechanism and non-membrane action mechanism);then, the highly efficient screening methods of AMP are systematically reviewed, including bacterial adsorption, cell membrane chromatography, phospholipid membrane chromatography, capillary electrophoresis, colorimetry, thin layer chromatography, fluorescence screening,high-throughput sequencing, and mining databases screening. In addition, the development direction of efficient exploration of AMP is also prospected. This paper provides a scientific basis for the discovery of AMPs from complex natural product systems.

关 键 词：抗微生物肽 天然产物 高效筛选 膜作用机制 

分 类 号：TS201.1[轻工技术与工程—食品科学]