外源腐胺(Put)处理对黄皮甜瓜果实采后冷害及活性氧代谢的影响  

Effect of Exogenous Putrescine(Put) Treatments on Postharvest Chilling Injury and Reactive Oxygen Metabolism of Yellow Melon Fruits

作　　者：徐斌[1,2,3] 安路明 孟新涛 张婷[1,2,3] XU Bin;AN Luming;MENG Xintao;ZHANG Ting(Research Institute of Farm Products Storage and Processing,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Xinjiang Intensive Processing Engineering Technology Research Center of Main Agricultural and Sideline Products,Urumqi 830091,China;Xinjiang Key Laboratory of Agro-products Processing and Preservation,Urumqi 830091,China)

机构地区：[1]新疆农业科学院农产品贮藏加工研究所,新疆乌鲁木齐830091 [2]新疆主要农副产品精深加工工程技术研究中心,新疆乌鲁木齐830091 [3]新疆农产品加工与保鲜重点实验室,新疆乌鲁木齐830091

出　　处：《食品工业科技》2022年第21期360-367,共8页Science and Technology of Food Industry

基　　金：国家自然基金项目“黄皮甜瓜低温/抗冷诱导过程中多胺(PAs)代谢和转录因子CmCBFs之间的关系研究(31860580)”。

摘　　要：为探讨外源腐胺(Put)处理减轻黄皮甜瓜果实采后低温冷害的调控机理,本试验以‘新密3号’黄皮甜瓜为试材,研究了0(CK)和2.0 mmol/L Put处理对其5±0.5℃低温贮藏期间果实冷害及活性氧代谢的影响。结果表明:低温诱导条件下,2.0 mmol/L外源Put处理可使黄皮甜瓜果皮中超氧化物歧化酶(SOD)活性保持较高的水平,使过氧化氢酶(CAT)活性、过氧化物酶(POD)活性和抗坏血酸过氧化物酶(APX)活性在贮藏21 d(严重冷害)时较CK分别提高2.3倍、1.6倍和1.2倍,达到30.06、2.46和39.14 U·gFW,显著抑制了贮藏28~49 d果皮中超氧阴离子自由基(O·~-)、过氧化氢(HO)的积累及脂氧合酶(LOX)活性和丙二醛(MDA)含量的上升,从而减轻活性氧自由基对细胞膜的伤害,使‘新密3号’黄皮甜瓜果实采后冷害发生症状减轻,发生时间推迟7 d,至贮藏结束时,冷害指数仅为0.27,显著低于对照处理。可见,外源腐胺(Put)处理减轻黄皮甜瓜冷害与其果皮组织活性氧代谢密切相关。In order to investigate the regulation mechanism that exogenous putrescine(Put) treatments on reducing chilling injury of postharvest yellow melon fruits, ’Xinmi No.3’ yellow melon fruits was used as material, and the effect that exogenous putrescine( 0 and 2.0 mmol/L Put) treatments on the chilling injury were studied and reactive oxygen metabolism of yellow melon fruits under 5±0.5 ℃ temperature. The results showed that 2.0 mmol/L Put treatment could maintain higher level of superoxide dismutase(SOD) activity, furthermore, it could increase the activity of catalase(CAT),peroxidase(POD) and ascorbate peroxidase(APX) 2.3, 1.6, 1.2 times, respectively compared with CK at 21 days(Severe chilling injury), and reached to 30.06, 2.46 and 39.14 U·gFW, respectively. It inhibited the accumulation of superoxide anion radical(O·) and hydrogen peroxide(HO), the activity of lipoxygenase(LOX) and the content of malondialdehyde(MDA) at 28~49 days significantly, thus, it could alleviate the damage of reactive oxygen species to cell membrane, and relieved the symptoms of chilling injury of ‘Xinmi No.3’ yellow melon fruits, delay the occurrence time of chilling injury by 7 d, the chilling injury index was only 0.27 until at the end of storage period, it lower than the control significantly.Therefore, the exogenous putrescine(Put) treatment can alleviate chilling injury of yellow melon is closely related to balance reactive oxygen metabolism.

关 键 词：外源腐胺 甜瓜 低温冷害 活性氧代谢 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]