Erianin inhibits oral cancer cell growth,migration,and invasion via the Nrf2/HO-1/GPX4 pathway  

作　　者：Xiu-Yun Tian Rui Han Qing-Yang Huang Mei-Yun Zhou Bin Luo Xin-Ru Chen Jin-Cheng Xu 

机构地区：[1]Department of Stomatology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui,China [2]Bengbu Medical College,Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals,Bengbu 233030,Anhui,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第10期437-445,共9页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To evaluate the effect of erianin on the viability,migration,and invasion of KB cells and elucidate its underlying mechanisms.Methods:Cell Counting Kit-8,colony formation,wound healing,and Transwell assays were used to determine the proliferation,migration,and invasion of oral cancer KB cells.Furthermore,malondialdehyde(MDA)and glutathione(GSH)levels were determined.Fluorescent probes were used to detect changes in intracellular reactive oxygen species and iron ions.Additionally,the expressions of ferroptosis-related proteins,NF-E2-related factor 2(Nrf2),ferritin heavy chain 1(FTH1),heme oxygenase 1(HO-1),and glutathione peroxidase 4(GPX4)were analyzed by Western blotting assays.Results:Erianin induced ferroptosis and inhibited the proliferation,migration,and invasion of KB cells.Moreover,erianin decreased GSH level,increased MDA level,elevated intracellular ROS and Fe2+contents,and downregulated the expression of the ferroptosis-related proteins Nrf2,HO-1,GPX4,and FTH1 in KB cells.These effects of erianin were effectively reversed by a ferroptosis inhibitor,ferrostatin-1.Conclusions:Erianin inhibits the proliferation,migration,and invasion of oral cancer cells and induces ferroptosis via the Nrf2/HO-1/GPX4 signaling pathway.Therefore,erianin may be a potential candidate for the treatment of oral cancer.

关 键 词：ERIANIN Ferroptosis Oral cancer Reactive oxygen species Therapy Nrf2/HO-1/GPX4 

分 类 号：R739.8[医药卫生—肿瘤]