AMP-activated protein kinaseβ1 orβ2 deletion enhances colon cancer cell growth and tumorigenesis  

作　　者：Fuli Shi Zhimin Tang Shanshan Jiang Zhijuan Xiong Wansi Zhang Yuanjun Li Hui Lin Zhijun Luo Ying Ying 

机构地区：[1]Jiangxi Medical Center for Major Public Health Events,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China [2]Jiangxi Province Key Laboratory of Tumor Pathogenesis and Molecular Pathology and Department of Pathophysiology,School of Basic Medical Sciences,Nanchang University,Nanchang 330006 China [3]Department of Immunobiology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China [4]Institute of Hematological Research,Shaanxi Provincial People’s Hospital,Xi’an 710000,China [5]Departments of Gastroenterology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第8期1140-1147,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.81572753);the Science and Technology Foundation of Jiangxi Province(No.20212BAB206039);an Innovation and Entrepreneurship Grant from Jiangxi Province Bureau of Foreign Experts.

摘　　要：Abnormal metabolism is a major hallmark of cancer and has been validated as a therapeutic target.Adenine monophosphate-activated protein kinase(AMPK),anαβγheterotrimer,performs essential functions in cancer progression due to its central role in maintaining the homeostasis of cellular energy.While the contributions of AMPKαand AMPKγsubunits to cancer development have been established,specific roles of AMPKβ1 and AMPKβ2 isoforms in cancer development are poorly understood.Here,we show the functions of AMPKβ1 and AMPKβ2 in colon cancer.Specifically,deletion of AMPKβ1 or AMPKβ2 leads to increased cell proliferation,colony formation,migration,and tumorigenesis in HCT116 and HT29 colon cancer cells.Interestingly,the AMPKβ1 and AMPKβ2 isoforms have slightly different effects on regulating cancer metabolism,as colon cancer cells with AMPKβ1 knockout showed decreased rates of glycolysis-related oxygen consumption,while AMPKβ2 deletion led to enhanced rates of oxygen consumption due to oxidative phosphorylation.These results demonstrate that functional AMPKβ1 and AMPKβ2 inhibit growth and tumorigenesis in colon cancer cells,suggesting their potential as effective targets for colon cancer therapy.

关 键 词：AMPKβ1 AMPKβ2 Warburg effect TUMORIGENESIS colon cancer 

分 类 号：R735.35[医药卫生—肿瘤]