红毛藻多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节机理  

Immunomodulatory Mechanism of Bangia fusco-purpurea Polysaccharides in Cyclophosphamide-Induced Immunosuppressed Mice

作　　者：吴靖娜 潘南[2] 陈晓婷[2] 陈贝[2] 刘智禹[2] WU Jingna;PAN Nan;CHEN Xiaoting;CHEN Bei;LIU Zhiyu(Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources,Xiamen Medical College,Xiamen 361023,China;Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province,Fisheries Research Institute of Fujian,Xiamen 361013,China)

机构地区：[1]厦门医学院,海洋生物医药资源福建省高校工程研究中心,福建厦门361023 [2]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013

出　　处：《食品科学》2022年第19期174-183,共10页Food Science

基　　金：福建省自然科学基金面上项目(2022J011398);福建省海洋生物增养殖与高值化利用重点实验室开放课题(2021fjscq13)。

摘　　要：本研究以环磷酰胺(cyclophosphamide,CTX)诱导的免疫力低下小鼠为模型,探究红毛藻多糖(Bangia fusco-purpurea polysaccharides,BFP)对免疫抑制小鼠免疫力的影响。结果表明,BFP干预能够显著提高免疫抑制小鼠自然杀伤细胞活性、巨噬细胞吞噬能力和清除碳颗粒能力;增强T淋巴细胞增殖作用,上调CD4^(+)T细胞比例,降低CD8+T细胞比例并增加CD4^(+)/CD8^(+),降低辅助性T细胞17和CD3^(-)CD19^(+)B细胞的比例;提高血清溶血素、免疫球蛋白(immunoglobulin,Ig)A和IgG、免疫相关细胞因子白细胞介素(interleukin,IL)-2、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素-γ(interferon-γ,INF-γ)的质量浓度,对免疫抑制小鼠的非特异性免疫、细胞免疫及体液免疫均具有良好的调节作用。在明确BFP对免疫抑制小鼠免疫功能具有保护作用的基础上,分析其对免疫抑制小鼠肠道相关免疫细胞表面受体的影响,发现BFP能够显著或极显著下调Toll样受体(Toll-like receptor,TLR)2、TLR4、IL-6和TNF-α基因(P<0.05)和蛋白(P<0.01)的表达,推测BFP通过TLR2/TLR4下游相关信号通路调节轴发挥增强免疫力的功能。本研究可为深入认识海洋食品的营养价值,推进海洋食品在居民膳食中的应用提供科学依据。The immunomodulatory effects of Bangia fusco-purpurea polysaccharides (BFP) in a mouse model of cyclophosphamide (CTX)-induced immunosuppression were investigated. As a result, BFP intervention significantly improved natural killer (NK) cell viability, macrophage phagocytosis and carbon clearance capacity, enhanced T-lymphocyte proliferation, up-regulated the proportions of CD4^(+), down-regulated the proportions of CD8^(+)T cells, increased the ratio of CD4^(+)/CD8^(+)cells, reduced the proportion of Th17 cells and the ratio of CD3^(-)CD19^(+)B cells, and increased the levels of serum hemolysin, immunoglobulin (Ig) A, IgG and immune-related cytokines such as interleukin (IL)-2, IL-6, tumor necrosis factor-α (TNF-α), and interferon-γ (INF-γ), which demonstrated its potent regulatory effects on nonspecific immunity, cellular immunity and humoral immunity in immunosuppressed mice. Furthermore, the effects of BFP on the surface receptors of intestinal immune cells in immunosuppressed mice were analyzed. It was found that BFP signicantly downregulated the mRNA (P < 0.05) and protein (P < 0.01) expression of Toll-like receptor 2 (TLR2), TLR4, IL-6 and TNF-α. Based on this, we speculated that BFP can enhance the immunity through the regulation axis of the TLR2/TLR4 downstream signaling pathway. This study provides scienti?c support for in-depth understanding of the nutritional value of sea foods and promoting the incorporation of sea foods into residents’ diets.

关 键 词：红毛藻多糖 细胞免疫 体液免疫 TOLL样受体 

分 类 号：TS201.4[轻工技术与工程—食品科学]