顺磁离子介导磁弛豫生物传感器评定鱼肉新鲜度  

Paramagnetic Ions-mediated Magnetic Relaxation Switching Biosensor for Fish Freshness Analysis

作　　者：曹子岳 董永贞 彭雪雯 陈瑞 江丰 范志勇 俞晓平 陈翊平[1] CAO Ziyue;DONG Yongzhen;PENG Xuewen;CHEN Rui;JIANG Feng;FAN Zhiyong;YU Xiaoping;CHEN Yiping(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Hubei Provincial Institute for Food Supervision and Test,Wuhan 430074,China;College of Life Sciences,China Jiliang University,Hangzhou 310018,China)

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]湖北省食品质量安全监督检验研究院,湖北武汉430074 [3]中国计量大学生命科学学院,浙江杭州310018

出　　处：《食品科学》2022年第20期304-312,共9页Food Science

基　　金：国家重点研发计划子课题(2019YFC1605502-3)。

摘　　要：构建一种新型的顺磁离子价态转换介导的磁弛豫生物传感方法,用于鱼肉中次黄嘌呤和组胺的快速检测,进而评定鱼肉的新鲜度和品质。通过利用次黄嘌呤和组胺被酶催化分解产生的过氧化氢的还原性和氧化性,诱导不同价态顺磁离子(Mn(VII)/Mn(II)和Fe(II)/Fe(III))的转化,使磁信号发生变化,实现次黄嘌呤和组胺的定量分析。结果表明:顺磁离子介导的磁弛豫生物传感器能够实现次黄嘌呤和组胺的高灵敏检测,次黄嘌呤和组胺的线性范围分别为0.525~1 050μmol/L和5~1 000μmol/L,检出限分别为0.17μmol/L和2.34μmol/L,且在鱼肉样本检测中与高效液相色谱法一致性良好。该传感器灵敏度高、检测速度快、成本低,在鱼肉及相关产品新鲜度评定和品质控制方面具有良好的应用潜力。A novel paramagnetic ions-mediated magnetic relaxation switching biosensor was developed to detect hypoxanthine and histamine for the evaluation of fish freshness and quality. With the catalysis of xanthine oxidase or diamine oxidase, hypoxanthine and histamine can be decomposed to generate hydrogen peroxide. Hydrogen peroxide can act as a reductant or oxidant to induce the conversion of paramagnetic ions of different valence(Mn(VII)/Mn(II) and Fe(II)/Fe(III)), leading to an obvious change in magnetic signals. Herein, paramagnetic ions and hydrogen peroxide were employed to fabricate a magnetic signal readout system for the quantitative analysis of hypoxanthine and histamine. As a result, this biosensor allowed highly sensitive detection of hypoxanthine and histamine. The linear ranges for hypoxanthine and histamine were 0.525–1 050 and 5–1 000 μmol/L, the limits of detection were 0.17 and 2.34 μmol/L, respectively. The results of this biosensor for crucian carp samples were in good agreement with those of high performance liquid chromatography(HPLC). The proposed biosensor is sensitive, rapid, low cost and promising for fish freshness detection and quality control.

关 键 词：次黄嘌呤 组胺 鱼肉新鲜度 顺磁离子 磁弛豫生物传感器 

分 类 号：TS201.6[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程] TS207.7