溶剂-超声波-酶法辅助提取桑葚果渣色素及其抗氧化活性研究  

Mulberry Residue Pigment Extracted by Assisted by Solvent-Ultrasonic-Enzyme Method and Its Antioxidant Activity

作　　者：吴均 吴俊葶 黄传书 赵珮 刘艳 马婧秋 WU Jun;WU Jun-ting;HUANG Chuan-shu;ZHAO Pei;LIU Yan;MA Jing-qiu(Chongqing Sericulture Science and Technology Research institute,Chongqing 400700,China;Army Logistics University of PLA,Chongqing 401331,China)

机构地区：[1]重庆市蚕业科学技术研究院,重庆400700 [2]中国人民解放军陆军勤务学院,重庆401331

出　　处：《食品研究与开发》2022年第21期123-130,共8页Food Research and Development

基　　金：重庆市科研院所绩效激励引导专项(scky2020jxjlsc0401)。

摘　　要：以桑葚果渣粉末为原料,在单因素试验的基础上,采用响应面试验设计对桑葚果渣色素的提取工艺进行优化,并研究桑葚果渣色素提取液的抗氧化活性。结果表明:在料液比1∶28(g/mL)、7%柠檬酸-90%乙醇(体积比1∶1)、纤维素酶添加量80 U/mL、超声温度48℃、超声时间41 min、超声功率300 W的条件下,桑葚果渣色素的提取效果最佳;提取液对2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸阳离子[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)cation,ABTS+]自由基和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基均具有清除作用,140μL桑葚果渣色素提取液的DPPH自由基清除率为97.72%,60μL桑葚果渣色素提取液的ABTS+自由基清除率为98.22%。This study took mulberry residue powder as the raw materials to optimize the extraction techniques of mulberry residue pigment by response surface test based on single factor test and studied the antioxidant activity of mulberry residue extracts. The results showed that the optimum conditions to extract mulberry residue pigment were 1 ∶ 28(g/mL)of the liquid ratio,7% citric acid-90% ethanol(1 ∶ 1 of the volume ratio),80 U/mL cellulase,and ultrasonically extracting for 41 min under 300 W at 48 ℃. The extracts under such conditions had the scavenging effect on 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)cation(ABTS+)free radicals and1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals. The DPPH radical scavenging rate was 97.72%by 140 μL mulberry residue pigment extracts and the ABTS+radical scavenging rate was 98.22% by 60 μL mulberry residue pigment extracts.

关 键 词：桑葚果渣 溶剂-超声波-酶法 提取 工艺优化 抗氧化活性 

分 类 号：TS264.4[轻工技术与工程—发酵工程] TS209[轻工技术与工程—食品科学与工程]