新型生物催化剂催化合成植物甾醇酯工艺优化  

Process Optimization for the Synthesis of Phytosterol Esters Catalyzed by A Novel Biocatalyst

作　　者：刘鑫龙[1] 王立晖[1] LIU Xin-long;WANG Li-hui(Department of Biochemical Engineering,Tianjin Modern Vocational Technology College,Tianjin 300350,China)

机构地区：[1]天津现代职业技术学院生物工程学院,天津300350

出　　处：《食品研究与开发》2022年第21期131-137,共7页Food Research and Development

基　　金：天津市科技计划项目(20YDTPJC01580)。

摘　　要：以具有3D开放孔隙特征的介孔有机硅球为载体构建新型生物催化剂,并用于催化合成植物甾醇酯。采用单因素试验,考察底物类型、底物浓度、酶用量、温度和反应时间5个因素对植物甾醇转化率的影响。通过响应面分析法确定植物甾醇酯的最佳合成条件,当棕榈酸(27.5 mmol/L)作为底物、酶用量21.4 U、反应温度47.2℃、反应时间11.4 h时,植物甾醇获得最大转化率为(96.8±0.7)%。此外,催化剂重复使用10次后,甾醇转化率为97.11%,表明该催化剂具有良好的稳定性。A novel biocatalyst was constructed with mesoporous organosilica spheres(with 3D open channels)as support and used for the synthesis of phytosterol esters. The effects of substrate type,substrate concentration,enzyme dosage,reaction temperature,and reaction time on the conversion of phytosterol were investigated by single factor tests. The synthesis conditions of phytosterol esters were optimized by response surface methodology. The highest conversion of phytosterols was(96.8±0.7)% in the case of 27.5 mmol/L palmitic acid as substrate,enzyme dosage of 21.4 U,the reaction temperature at 47.2 ℃ and the reaction time of 11.4 h. In addition,the sterol conversion rate was 97.11% after the biocatalyst was reused for 10 times,which demonstrated that the biocatalyst owned good stability.

关 键 词：介孔有机硅球 3D开放孔道 植物甾醇酯 皱褶假丝酵母脂肪酶 响应面法 

分 类 号：TS202.1[轻工技术与工程—食品科学] TQ426.97[轻工技术与工程—食品科学与工程]