苦瓜多糖调节宿主免疫应答缓解右旋糖酐硫酸钠(DSS)诱导的小鼠结肠炎  

Momordica charantia polysaccharide modulates host immune response and alleviates DSS-induced colitis in mice

作　　者：罗天池 吴小小 胡君怡 姚均存 卢景怡 黄孜玲 杨欢 Luo Tianchi;Wu Xiaoxiao;Hu Junyi;Yao Juncun;Lu Jingyi;Huan Ziling;Yang Huan(School of Medical Technology,Xuzhou Medical University,Xuzhou 221004,China)

机构地区：[1]徐州医科大学医学技术学院,徐州221004

出　　处：《中华微生物学和免疫学杂志》2022年第9期722-728,共7页Chinese Journal of Microbiology and Immunology

基　　金：江苏省高等学校大学生实践创新训练计划一般项目(202110313080Y)。

摘　　要：目的探究苦瓜多糖(Momordica charantia polysaccharide,MCP)对右旋糖酐硫酸钠(dextran sulfate sodium,DSS)诱导的小鼠溃疡性结肠炎(ulcerative colitis,UC)的保护作用及机制。方法提取MCP,用其治疗DSS诱导的UC。将小鼠随机分为对照组、DSS组和DSS+MCP组,每组5只,每天监测小鼠的体重和疾病活动指数(disease activity index,DAI)。收集小鼠结肠组织和血清,采用HE染色、ELISA、RT-qPCR和流式细胞术分析肠道组织、炎症因子、CD4^(+)T细胞、中性粒细胞和巨噬细胞变化。结果MCP通过恢复小鼠体重和大便黏稠度降低DAI,减少出血,从而改善DSS诱导的UC。另外,MCP能够修复结肠组织的黏膜屏障功能,降低炎症细胞浸润,减轻黏膜层和肌肉层水肿,保护UC小鼠的肠道。MCP处理后小鼠结肠组织中炎症因子TNF-α和IL-1β的表达水平降低,CD4^(+)T细胞水平也降低。结论MCP通过抑制小鼠体重下降、修复结肠组织损伤、改善免疫系统紊乱、抑制DSS诱导的炎症细胞因子水平升高等途径改善DSS诱导的UC。本研究为MCP作为UC治疗的一种潜在饮食干预提供了参考。Objective To investigate the protective effect of Momordica charantia polysaccharide(MCP)on dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)in mice and the possible mechanism.Methods MCP was extracted from Momordica charantia(MC).Fifteen C57BL/6J mice were randomly divided into three groups with five in each group:control group,DSS group and DSS+MCP group.The body weight and disease activity index(DAI)of the mice were monitored every day.Mouse colon tissues and serum samples were collected.Pathological changes in intestinal tissues and the expression of inflammatory factors,CD4^(+)T cells,neutrophils and macrophages were analyzed by HE staining,ELISA,RT-qPCR and flow cytometry.Results MCP alleviated the DSS-induced UC in mice by restoring body weight and stool consistency and reducing bleeding.Moreover,MCP could repair the mucosal barrier function of colon tissues,decreasing inflammatory cell infiltration and lessening the edema in mucosal layer and muscle layer,and therefore protect the damaged intestinal tract of mice.The expression of inflammatory cytokines(TNF-αand IL-1β)and the level of CD4^(+)T cells were decreased in the colonic tissues of MCP-treated mice.Conclusions MCP ameliorated DSS-induced UC in mice through inhibiting weight loss,repairing colonic tissue damage,improving immune system disorder and decreasing the expression of inflammatory cytokines.This study provided reference for further study of MCP as a potential dietary intervention in the treatment of UC.

关 键 词：苦瓜多糖 溃疡性结肠炎 免疫应答 

分 类 号：R574.62[医药卫生—消化系统]