麦芽糖淀粉酶CDS 1-3在淀粉糖化过程中的作用分析  

Analysis of the Role of Maltogenic Amylase CDS 1-3 in the Process of Starch Saccharification

作　　者：魏瑞宣 管明东 王宏萌 汤宏赤 刘艺 林丽华[2] 郭媛[2] 王子龙 庞浩[1,2] WEI Ruixuan;GUAN Mingdong;WANG Hongmeng;TANG Hongchi;LIU Yi;LIN Lihua;GUO Yuan;WANG Zilong;PANG Hao(College of Life Science and Technology,Guangxi University,Nanning,Guangxi,530005,China;State Key Laboratory of Non-Food Biomass and Enzyme Technology,National Engineering Research Center for Non-Food Biorefinery,Guangxi Key Laboratory of Biorefinery,Guangxi Academy of Sciences,Nanning,Guangxi,530007,China)

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]广西科学院,国家非粮生物质能源工程技术研究中心,非粮生物质酶解国家重点实验室,广西生物炼制重点实验室,广西南宁530007

出　　处：《广西科学院学报》2022年第3期260-266,共7页Journal of Guangxi Academy of Sciences

基　　金：广西重大科技创新基地建设项目(2018-15Z03-1206);广西重点研发计划(AB17195034)资助。

摘　　要：为探究多糖相互作用对淀粉糖化速率的影响,本研究使用较传统麦芽糖淀粉酶更具支链淀粉水解能力的麦芽糖淀粉酶CDS 1-3,将其与α-淀粉酶及糖化酶协同进行淀粉糖化,并对糖化液进行还原糖测定以及高效液相色谱分析。结果表明:反应到30 min时,CDS 1-3作用效果最明显,相比于Control组,CDS 1-3组糖化液的葡萄糖当量(Dextrose Equivalent, DE)值提高了4.51%。随着反应的进行,DE值提高速率降低。反应到60 min时,DE值提高了1.17%;反应到90 min时,DE值提高了2.12%。高效液相色谱结果显示,在整个反应过程中,CDS 1-3组葡萄糖和麦芽糖的产量始终高于Control组,说明CDS 1-3可有效加快木薯淀粉糖化速率,可应用于淀粉工业中。To investigate the effect of multi-enzyme interaction on starch saccharification rate, maltogenic amylase CDS 1-3,which was more capable of amylopectin hydrolysis than traditional maltogenic amylase, was combined with α-amylase and glucoamylase for starch saccharification, and the reducing sugar in saccharification liquid was determined and analyzed by high performance liquid chromatography in this study.The results showed that when the reaction was 30 min, the effect of CDS 1-3 was the most obvious.Compared with the Control group, the Dextrose Equivalent(DE) value of the saccharification solution in the CDS 1-3 group was increased by 4.51%.As the reaction proceeded, the rate of increase in DE value decreased.When the reaction was 60 min, the DE value was increased by 1.17%;when the reaction was 90 min, the DE value was increased by 2.12%.The results of high performance liquid chromatography showed that the yields of glucose and maltose in the CDS 1-3 groups were always higher than those in the Control group during the whole reaction process, which indicated that CDS 1-3 could effectively accelerate the saccharification rate of cassava starch and could be used in the starch industry.

关 键 词：麦芽糖淀粉酶 木薯淀粉 支链淀粉 支链淀粉水解酶 协同糖化 

分 类 号：Q556[生物学—生物化学]