Investigating the cytotoxic effect of ibuprofen concentration in liver cancer cells(HepG2)and normal fibroblast(AGO)  

作　　者：Sanaz Pashapour Abbas Zabihi Roya Behrouzi 

机构地区：[1]Department of Pharmacology and Toxicology,Faculty of Pharmacy and Pharmaceutical Sciences,Tehran Medical Sciences,Islamic Azad University,Tehran 009821,Iran [2]Department of Biology,Faculty of Basic Sciences,Islamic Azad University,Hamedan Branch,Hamedan 009881,Iran [3]Department of Food Industries,Islamic Azad University,Tehran 009821,Iran

出　　处：《Toxicology Advances》2022年第4期1-4,共4页毒理学前沿

摘　　要：Objective:Although many studies have reported that nonsteroidal anti-inflammatory drugs can have anticancer effects,the results are still challenging.The aim of this research is to Mechanism and effect of anti-inflammatory drugs in cancer treatment.Methods:In this laboratory study,cell lines were randomly divided into control group(no exposure to ibuprofen and groups exposed to ibuprofen concentrations of 10,1,0.1,and 0.001 mg/mL.The cytotoxic effect of ibuprofen was measured at 24 and 72 hours using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT).Data were compared between groups using a one-way variance test.Results:The results showed that the viability rate of HepG2 cancer cells at concentrations of 1 and 10 mg/mL decreased significantly compared to the control group in 24 hours(P<0.0001).Also,the viability rate at concentrations of 1,10,0.1,and 0.001 mg/mL decreased significantly compared to the control group in 72 h(P<0.0001).Only the concentration of 10 mg/mL ibuprofen decreased the viability of normal cells compared to the control(P<0.05).Conclusion:Overall,the results of this research showed that different concentrations of ibuprofen had a cytotoxic effect on liver cancer cells,and except for the concentration of 10 mg/mL,the other concentrations did not have a cytotoxic effect on normal cells.

关 键 词：liver cancer cells IBUPROFEN non-steroidal anti-inflammatory drugs CYTOTOXIC 

分 类 号：R73[医药卫生—肿瘤]