无子刺梨的体内外抗氧化活性及化学成分分析  

Antioxidant Activities and Content Analysis of Chemical Constituents in Rosa sterilis

作　　者：安雪菲 但汉龙[1] 罗旭璐 刘云[1] 李永和 赵平[1] An Xuefei;Dan Hanlong;Luo Xulu;Liu Yun;Li Yonghe;Zhao Ping(Key Laboratory of National Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650233,China;Yunnan Forestry Technological College,Kunming Yunnan 650224,China)

机构地区：[1]西南林业大学西南地区林业生物质资源高效利用国家林业局重点实验室,云南昆明650233 [2]云南林业职业技术学院,云南昆明650224

出　　处：《西南林业大学学报（自然科学）》2022年第5期177-182,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：推进黔西南试验区建设科技合作专项资金项目(黔西南科合2015−31)资助;西南林业大学科技创新基金项目(C15081)资助。

摘　　要：采用DPPH和ABTS自由基清除活性和ROS清除活性测试方法,评价无子刺梨果汁及果渣提取物的体内外抗氧化活性,采用紫外分光光度法分别对果汁和果渣中多酚、黄酮和三萜等主要化学成分的含量进行测定分析。结果表明:果汁提取物对DPPH自由基和ABTS自由基的半清除浓度分别为(17.58±0.48)μg/mL和(56.26±3.07)μg/mL,果渣提取物对DPPH和ABTS自由基的半清除浓度分别为(45.38±0.68)μg/mL和(101.67±1.15)μg/mL,果汁提取物的体外抗氧化活性均强于阳性对照Vc和果渣提取物。果汁提取物和果渣提取物的ROS清除率分别为(84.80±3.40)%和(83.80±3.60)%,表明2种提取物的体内抗氧化活性相当,且均强于阳性对照。果汁中总多酚、总黄酮和总三萜的含量分别为(32.81±0.19)、(29.62±0.06)、(61.88±0.62)mg/g,果渣中总多酚、总黄酮和总三萜的含量分别为(21.66±0.29)、(12.11±0.05)、(15.16±0.22)mg/g,果汁中主要化学成分的含量均高于果渣,并与其抗氧化能力呈正相关关系。In order to evaluate the antioxidant activities and the major chemical constituents of Rosa sterilis,the antioxidant activities of the juice extract and pomace extract of title plant were evaluated by DPPH and ABTS free radical scavenging assays,and the contents of total polyphenols,total flavonoids and total triterpenoids in the juice and pomace were determined by ultraviolet spectrophotometry,respectively.The results showed that the SC50 values of the juice extract against DPPH and ABTS free radicals were(17.58±0.48)μg/mL and(56.26±3.07)μg/mL,and the SC50 values for the pomace extract against DPPH and ABTS free radicals were(45.38±0.68)μg/mL and(101.67±1.15)μg/mL,respectively,indicated that the in vitro antioxidant activity of the juice extracts is stronger than those of the Vc and pomace extract.The ROS scavenging rates of juice and pomace extracts were(84.80±3.40)%and(83.80±3.60)%,respectively,indicated that the in vivo antioxidant activity of 2 extracts was similar and stronger than that of the positive control GSH.The results also showed that the contents of total polyphenols,total flavonoids and total triterpenoids were(32.81±0.19)mg/g,(29.62±0.06)mg/g and(61.88±0.62)mg/g in the juice,and(21.66±0.29)mg/g,(12.11±0.05)mg/g and(15.16±0.22)mg/g in the pomace,respectively,indicated the contents of the major chemical constituents in the juice is higher than those of the pomace,which with a positive correlation to their antioxidant activities.

关 键 词：无子刺梨 抗氧化活性 总多酚 总黄酮 总三萜 

分 类 号：Q946.8[生物学—植物学]