刺梨的活性成分含量分布与光谱表征  

Phytochemical Active Composites in Rosa Roxburghii Tratt.: Content Distribution and Spectroscopic Characterization

作　　者：陈家敏 李博岩 胡芸 张进 汪瑞敏 孙晓红[1] CHEN Jia-min;LI Bo-yan;HU Yun;ZHANG Jin;WANG Rui-min;SUN Xiao-hong(School of Public Health/Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 550025,China;Technology Center,China Tobacco Guizhou Industrial Co.Ltd.,Guiyang 550009,China)

机构地区：[1]贵州医科大学公共卫生与健康学院/环境污染与疾病监控教育部重点实验室,贵州贵阳550025 [2]贵州中烟工业有限责任公司技术中心,贵州贵阳550009

出　　处：《光谱学与光谱分析》2022年第11期3403-3408,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21864008,22004022);贵州省科技计划支持项目(黔科合基础[2018]1130,黔科合基础-ZK[2021]045);贵阳市科技计划资助项目(筑科合同[2017]30-27);贵州省区域内一流学科建设项目-公共卫生与预防医学(黔教科研发[2017]85)资助。

摘　　要：刺梨是蔷薇科蔷薇属多年生落叶灌木,其果实富含多种生物活性物质,具有重要的药食用价值。采用近红外、紫外-可见、激发发射三维荧光光谱技术系统性表征刺梨鲜果提取物化学组成,探讨不同产地的301个批次刺梨果中总酚、总黄酮、总三萜类物质的含量与自由基清除能力、铁离子还原能力等抗氧化活性的分布特征。结果显示刺梨果中具有高含量的酚类、黄酮类、三萜类物质,分别为9.23~37.45,8.80~27.96和6.91~22.62 mg·g^(-1) FW(新鲜刺梨果的重量)。刺梨果具有较好的自由基清除活性和还原能力,对1,1-二苯基-2-三硝基苯肼(DPPH)清除率为14.39%~83.19%、2,2’-联氮基-双-(3-乙基-苯并噻唑啉-6-磺酸)二铵盐(ABTS)清除率18.50%~68.45%、对铁离子还原能力(FRAP)0.08~0.44 mmol·L^(-1) TE·g^(-1) FW。这些物质含量与活性指标数据均服从正态分布,表明实验用的多批次刺梨样本具有多样性、代表性、随机性,并且不同产地的样本提取物的活性成分含量及抗氧化活性没有显著的统计学差异性。提取物样本的紫外-可见、近红外、荧光光谱都具有明显的谱带特征,其光谱数据的主判别变量模型可有效鉴别八个不同产地的刺梨样本。研究表明多光谱技术能够表征提取物的物质组成,可对刺梨鲜果进行快速检测、品质差异判别与产地溯源,为刺梨质量评价、选种和资源开发提供参考。植物提取物的总酚、总黄酮等活性成分含量、抗氧化活性等指标不能准确反映样本的活性物质组成特征性,不能用于样本产地溯源分析。Rosa roxburghii Tratt.(RRT)is a perennial deciduous shrub of Rosa genus in Rosaceae.Its fruit has specific nutritional and medicinal values due to a variety of bioactive ingredients present in a substantial amount.This study used NIR,UV-Vis and fluorescence spectroscopies to characterize the chemical composites of the RRT fruit extracts in 301 batches from different planting origins.Meanwhile,the distribution characteristics of the contents of total phenols(TPC),total flavonoids(TFC),and total triterpenoids(TTC)in the extracts were not only discussed,but also the antioxidant activities concerning free radical scavenging capacity and ferric reducing antioxidant power,concerning the DPPH,ABTS and FRAP assays.Results showed high TPC,TFC and TTC in RRT fruit,as were 9.23~37.45,8.80~27.96 and 6.91~22.62 mg·g^(-1) FW,respectively,which possibly led to a pretty good free radical scavenging capacity and reducing power.The scavenging rate of DPPH ranged between 14.39%~83.19%,the scavenging rate of ABTS was 18.50%~68.45%,and FRAP varied in 0.08~0.44 mmol·L^(-1) TE·g^(-1) FW.The statistical analysis indicated that all the individual TPC,TFC,TTC,DPPH,ABTS,and FRAP values from 301 batches were normally distributed.The samples used in the study might be pooled from the population and thus diverse,representative,and random.There was no significant statistical difference in the contents of active composites and antioxidant activities of the extracts regardless of the planting origins.All the UV-Vis-NIR and fluorescence spectra had characteristic bands.The resultant principal discriminant variate(PDV)models were able to identify the samples from eight planting origins from each other.When combined with the PDV models the UV-Vis,NIR and fluorescence spectroscopies could be used for compositional characterization,rapid detection and discrimination of the extracts.This work provided a new idea for the quality evaluation,strain selection and resource development of RRT,among other medicinal-edible plants.However,it was neither rel

关 键 词：刺梨 抗氧化活性 近红外光谱 激发发射荧光光谱 主判别变量法 

分 类 号：O657.3[理学—分析化学]