英红九号红茶水提物通过调控TNF-α/NF-κB信号通路缓解小鼠的急性酒精中毒  

Alleviating Acute Alcohol Intoxication in Mice using Yinghong No.9 Black Tea Extracts to Regulate the TNF-α/NF-κB Signaling Pathway

作　　者：赖幸菲[1] 王琼[2] 黎秋华[1] 孙伶俐[1] 陈若虹 文帅 张镇标 刘晓慧[2] 孙世利[1] LAI Xingfei;WANG Qiong;LI Qiuhua;SUN Lingli;CHEN Ruohong;WEN Shuai;ZHANG Zhenbiao;LIU Xiaohui;SUN Shili(Tea Research Institute,Guangdong Academy of Agricultural Sciences,Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation&Utilization,Guangzhou 510640,China;.College of Tea Science,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]广东省农业科学院茶叶研究所,广东省茶树资源创新利用重点实验室,广东广州510640 [2]云南农业大学茶学院,云南昆明650201

出　　处：《现代食品科技》2022年第10期1-8,共8页Modern Food Science and Technology

基　　金：广东省农业科学院农业优势产业学科团队建设项目(202126TD);国家自然科学基金青年科学基金项目(32060702);广东省基础与应用基础研究基金项目(2021A1515010958;2020A1515011266);科技创新战略专项资金(高水平农科院建设)项目(R2019PY-JX004);清远市科技计划项目(2020KJJH042);湛江市科技计划项目(2020A03014)。

摘　　要：该研究探讨了英红九号红茶水提物(Yinghong NO.9 Black Tea Water Extract,YBTE)缓解小鼠急性酒精中毒作用(Acute Alcoholic Intoxication,AAI)及其机制。采用56°白酒稀释液一次性灌胃方法建立小鼠AAI模型,记录翻正反射消失和恢复时间,检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性以及血清和肝脏中乙醇脱氢酶(ADH)的活性,采用Western blot法测定肝脏中核因子-κΒ(NF-κB)、炎症因子肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)蛋白表达水平。结果表明,不同剂量YBTE均能延长小鼠入睡时间,也能缩短睡眠时间,其中高剂量组小鼠入睡时间延长到40.89 min(p<0.01),睡眠时间缩短为166.18 min(p<0.01)。与模型组相比,YBTE可降低血清中ALT和AST的活性,提高血清和肝脏中ADH的活性,其中高剂量组ALT活性下降到22.89 U/L(p<0.01),AST活性下降到29.58 U/L(p<0.01),血清ADH提高到43.43 IU/mL(p<0.01),肝脏ADH提高到7.37 IU/mL(p<0.01),并能下调肝脏中p-NF-κB p65、TNF-α和IL-1β蛋白表达量。YBTE具有良好的缓解AAI的作用,其作用机制可能是促进酒精代谢和下调TNF-α/NF-κB信号通路。To study the effects and mechanisms of Yinghong No.9 black tea water-extract(YBTE)for alleviating acute alcohol intoxication(AAI),mice were subjected to one-time intragastric administration of 56°Baijiu(Chinese Liquor)to establish an AAI model.Subsequently,the time required to lose and regain the righting reflex of the mice were recorded.The alanine transaminase(ALT)and glutamic oxaloacetic transaminase(AST)activities in the serum and alcohol dehydrogenase(ADH)activity both in the serum and liver were measured.Moreover,the expression the nuclear factor kappa B(NF-κB),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)proteins in the liver were measured using western blot analyses.The results showed that sleep onset latency increased and sleep duration decreased for mice treated with various doses of YBTE compared with the model group.More specifically,the sleep onset latency for the high-dose YBTE-treated group increased to 40.89 min(p<0.01)and their sleep duration was 166.18 min(p<0.01).Moreover,the serum activities of ALT and AST were significantly lower and the ADH activity in the serum and liver were both significantly higher in the YBTE-treated groups than in the control group.In particular,the ALT and AST activities in the high-dose group decreased to 22.89 U/L(p<0.01)and 29.58 U/L(p<0.01),respectively,and the ADH activity in the serum and liver increased to 43.43 IU/mL(p<0.01)and 7.37 IU/mL(p<0.01),respectively.Furthermore,the expression of p-NF-κB p65,TNF-α,and IL-1βin the liver were significantly down regulated.In conclusion,AAI can be effectively relieved with YBTE by promoting ethanol metabolism and down regulating the TNF-α/NF-κB signaling pathway.

关 键 词：红茶水提物 急性酒精中毒 NF-ΚB信号通路 炎症 

分 类 号：TS272.52[农业科学—茶叶生产加工]