三酶两步水解法对杂粮代餐粉的品质特性改善  

Improvement of Quality Properties of Coarse Cereal Meal Replacement Powder by Three-enzyme Two-step Hydrolysis

作　　者：陈悦 高路 CHEN Yue;GAO Lu(College of Grain,Shenyang Normal University,Shenyang 110034,Liaoning,China)

机构地区：[1]沈阳师范大学粮食学院,辽宁沈阳110034

出　　处：《食品研究与开发》2022年第22期129-137,共9页Food Research and Development

基　　金：辽宁省教育厅2021年度高等学校基本科研项目(LJKZ0998)。

摘　　要：以紫薯为主要原料,辅以黄豆、花生仁、芝麻籽、玉米、小米、糙米、藜麦、薏米、青稞,选取纤维素酶和复合酶(α-淀粉酶、糖化酶)以三酶两步水解法制备杂粮代餐粉(coarse cereals meal replacement powder,CCMRP)。以葡萄糖当量(dextrose equivalent value,DE值)为指标,通过单因素试验,考察复合酶体积比、酶解时间、酶解温度、复合酶添加量对CCMRP的DE值影响。在单因素试验基础上,通过正交试验对酶解条件进一步优化。研究结果表明,CCMRP酶解条件的影响因素主次顺序:酶解温度>复合酶添加量>酶解时间>复合酶体积比,最佳酶解条件:复合酶体积比1∶2,酶解时间130 min,酶解温度55℃,复合酶添加量0.75%,此时DE值和冲调性的综合分最高,制得的CCMRP冲调性好,色泽、风味、口感俱佳,产品的填充性和流动性有所改善,更利于产品包装。A three-enzyme(cellulase and a compound enzyme composed ofα-amylase and glucoamylase)two-step hydrolysis method was employed to prepare the coarse cereal meal replacement powder(CCMRP)with purple sweet potato as the main raw material and supplemented with soybean,peanut,sesame,maize,foxtail millet,brown rice,quinoa,semen coicis,and highland barley.The effects of enzyme volume ratio,enzymolysis time and temperature,and enzyme addition amount on the dextrose equivalent(DE)value of CCMRP were investigated by single factor experiments.On the basis of single factor experiments,the enzymolysis conditions were optimized by orthogonal design.The results showed that the factors affecting the DE value of CCMRP followed the order of enzymolysis temperature>enzyme addition amount>enzymolysis time>enzyme volume ratio.The enzymolysis conditions were optimized as follows:enzyme volume ratio of 1∶2,enzymolysis at 55℃for 130 min,and enzyme addition amount of 0.75%.The CCMRP prepared under these conditions had the highest DE value,fast dissolvability,and excellent color,flavor,and taste.This preparation method improved the filling property and flowability of product,which was conducive to product packaging.

关 键 词：杂粮代餐粉 复合酶法 酶解条件 葡萄糖当量 品质特性 

分 类 号：TS217[轻工技术与工程—粮食、油脂及植物蛋白工程]