桑叶蛋白提取工艺优化及其酶解蛋白体外抗氧化活性  

Optimization of the Extraction Process and Antioxidant Activity of Mulberry Leaf Protein in vitro

作　　者：赵荣生[1] 孙锡南 王鑫 王宗成 ZHAO Rong-sheng;SUN Xi-nan;WANG Xin;WANG Zong-cheng(College of Innovation and Entrepreneurship,Hunan University of Science and Engineering,Yongzhou 425199,Hunan,China;Hunan Provincial Engineering Research Center for Comprehensive Development and Utilization of Biomass Resources,College of Chemical and Biological Engineering,Hunan University of Science and Engineering,Yongzhou 425199,Hunan,China)

机构地区：[1]湖南科技学院创新创业学院,湖南永州425199 [2]湖南科技学院化学与生物工程学院湖南省生物质资源综合开发利用工程技术研究中心,湖南永州425199

出　　处：《食品研究与开发》2022年第22期138-144,152,共8页Food Research and Development

基　　金：湖南省教育厅科学研究项目(20A206);湖南省科技创新人才计划资助项目(2021RC1014);湖南省高校优秀思想政治工作者项目(湘人才发[2021]9号No.40)。

摘　　要：为提高桑叶蛋白的得率和加强桑叶蛋白的开发利用,采用超声细胞破碎法提取桑叶蛋白,并研究桑叶酶解蛋白的体外抗氧化活性。考察浸提液的pH值、液料比、超声功率、破碎时间、浸提温度、浸提时间、浸提次数7个单因素对桑叶蛋白得率的影响,并采用正交试验设计优化提取工艺。采用木瓜蛋白酶酶解桑叶蛋白,得到桑叶酶解蛋白,以DPPH自由基清除法和总还原能力的测定方法评价桑叶酶解蛋白体外抗氧化活性强弱。结果表明,最佳提取工艺条件:浸提液的pH值为8,液料比为12∶1(mL/g),超声功率200 W下处理5 min,在30℃下水浴15 min浸提2次,在此条件下,桑叶蛋白的得率为9.85%。桑叶酶解蛋白具有一定的还原能力,且对DPPH自由基具有较强的清除能力。To improve the yield and explore the development and utilization of mulberry leaf protein,extracts were prepared from mulberry leaves using ultrasonic cell fragmentation,and the antioxidant activity of the extracted enzyme was studied in vitro.The effects of the pH value of the extraction liquid,the ratio of liquid to material,ultrasonic power,crushing time,extraction temperature,and extraction time on the yield of mulberry leaf protein were investigated.The extraction process was optimized using an orthogonal design.Enzymolysis of the mulberry leaf protein was performed using papain to obtain mulberry leaf enzyme.The antioxidant activities of the mulberry leaf enzyme in vitro were evaluated using the 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical scavenging method and the total reducing ability determination method.The results showed that the optimum extraction conditions were as follows:a pH value of 8 for the extraction liquid,a 12∶1(mL/g)extraction liquid to material ratio,5 min of ultrasonic treatment at a power of 200 W,an extraction temperature of 30℃,and extraction time of 15 min,and two extractions.The yield of mulberry leaf protein was 9.85%under these conditions.The mulberry leaf enzyme had reducing ability and a strong ability to scavenge DPPH free radicals.

关 键 词：桑叶蛋白 超声细胞破碎法 正交试验 酶解蛋白 抗氧化活性 

分 类 号：TQ936[化学工程]