Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor regulate the interaction between astrocytes and Schwann cells at the trigeminal root entry zone  

作　　者：Madeha Ishag Adam Ling Lin Amir Mahmoud Makin Xiao-Fen Zhang Lu-Xi Zhou Xin-Yue Liao Li Zhao Feng Wang Dao-Shu Luo 

机构地区：[1]Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province,Laboratory of Clinical Applied Anatomy,School of Basic Medical Sciences,Fujian Medical University,Fuzhou,Fujian Province,China [2]Public Technology Service Center of Fujian Medical University,Fuzhou,Fujian Province,China [3]Center for Membrane and Water Science&Technology,Institute of Oceanic and Environmental Chemical Engineering,Zhejiang University of Technology,Hangzhou,Zhejiang Province,China

出　　处：《Neural Regeneration Research》2023年第6期1364-1370,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Sclence Foundation of China in 2021;No.82171213;the Natural Science Foundation of Fujian Province in 2019;No.2019J01289 (both to DSL)

摘　　要：The trigeminal root entry zone is the zone at which the myelination switches from peripheral Schwann cells to central oligodendrocytes.Its special anatomical and physiological structure renders it susceptible to nerve injury.The etiology of most primary trigeminal neuralgia is closely related to microvascular compression of the trigeminal root entry zone.This study aimed to develop an efficient in vitro model mimicking the glial environment of trigeminal root entry zone as a tool to investigate the effects of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor on the structural and functional integrity of trigeminal root entry zone and modulation of cellular interactions.Primary astrocytes and Schwann cells isolated from trigeminal root entry zone of postnatal rats were inoculated into a two-well silicon culture insert to mimic the trigeminal root entry zone microenvironment and treated with glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor.In monoculture,glial cell line-derived neurotrophic factor promoted the migration of Schwann cells,but it did not have effects on the migration of astrocytes.In the co-culture system,glial cell line-derived neurotrophic factor promoted the bidirectional migration of astrocytes and Schwann cells.Brain-derived neurotrophic factor markedly promoted the activation and migration of astrocytes.However,in the co-culture system,brain-derived neurotrophic factor inhibited the migration of astrocytes and Schwann cells to a certain degree.These findings suggest that glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor are involved in the regulation of the astrocyte-Schwann cell interaction in the co-culture system derived from the trigeminal root entry zone.This system can be used as a cell model to study the mechanism of glial dysregulation associated with trigeminal nerve injury and possible therapeutic interventions.

关 键 词：ASTROCYTES brain-derived neurotrophic factor cell migration glial cell line-derived neurotrophic factor glial interaction Schwann cells trigeminal nerve 

分 类 号：R741[医药卫生—神经病学与精神病学]