胶原蛋白肽复合银耳多糖对D-半乳糖致衰小鼠的改善作用  

The Improving Effect of the Complex of Collagen Peptides Combined with Tremella fuciformis Polysaccharides on D-galactose-induced Ageing Mice

作　　者：徐晓飞 赵明月 钟兴伟 何瑞琪 Xu Xiaofei;Zhao Mingyue;Zhong Xingwei;He Ruiqi(College of Food Science and Engineering,Guangdong Ocean University,Yangjiang 529599,Guangdong;South China Institute of Collaborative Innovation,Dongguan 523808,Guangdong;Geometry Cell Biology Research Center,Dongguan 523808,Guangdong)

机构地区：[1]广东海洋大学食品科学与工程学院,广东阳江529599 [2]华南协同创新研究院,广东东莞523808 [3]几何细胞生物研究中心,广东东莞523808

出　　处：《中国食品学报》2022年第10期134-142,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC1602106)。

摘　　要：目的:探讨胶原蛋白肽复合银耳多糖对D-半乳糖致衰小鼠老化皮肤的抗衰作用。方法:建立D-半乳糖衰老小鼠模型,分为模型组(灌胃生理盐水)、胶原蛋白肽(CP)组[830 mg/(kg·d)]以及胶原蛋白肽复合银耳多糖(CPTP)组[CP 830 mg/(kg·d)+银耳多糖100 mg/(kg·d)],同时设正常对照组(灌胃生理盐水),实验持续8周。实验结束处死小鼠,测定各组小鼠皮肤中羟脯氨酸和透明质酸含量、体内抗氧化酶活力、炎性因子(IL-1β、TNF-α)浓度及肠道内短链脂肪酸(SCFAs)水平。结果:CP组、CPTP组均可显著增加小鼠老化皮肤中羟脯氨酸和透明质酸水平(P<0.01),提高衰老小鼠血清、肝脏和心脏组织中GSH-Px、SOD活力,并降低MDA浓度(P<0.01),降低血清中IL-1β、TNF-α水平(P<0.01),然而对肠道内SCFAs水平无显著影响。CPTP在改善皮肤老化和降低血清炎性因子水平上,显著优于CP(P<0.01)。结论:相比单一CP,CPTP可更有效缓解皮肤老化,其作用机理可能是通过降低机体氧化压力和抑制炎症因子分泌而实现。本实验结果为胶原蛋白肽复合银耳多糖应用于美容功能食品提供数据支持。Objective:To investigate the anti-ageing effects of the complex of collagen peptides combined with Tremella fuciformis polysaccharides(CPTP)on skin aging in D-galactose induced aging animal model and its mechanism.Method:D-galactose-induced aging mouse model were divided into three groups:Model group(administration of physiological saline),CP group[administration of collagen peptide in a dose of 830 mg/(kg·d)],and CPTP group[administration of a complex of collagen peptide and T.fuciformis polysaccharides in a dose of 830 mg/(kg·d)and 100 mg/(kg·d),respectively].Adult mice were set as normal group.Intraperitoneal injection(i.p.)daily with physiological saline were conducted for mice in Normal group,while i.p.daily with D-galactose in a dosage of 120 mg/(kg·d)were performed for those in remaining groups.All mice were given intragastric administration for eight weeks and then killed.The concentrations of hydroxyproline(HYP)and hyaluronic acid(HA)in skin,the activities of anti-oxidant enzymes and the production of MDA in tissues,the contents of inflammatory cytokines in serum,the productions of intestinal SCFAs as well as the pH value were measured.Results:CPTP remarkably improved the contents of HYP and HA in skin(P<0.01)and its efficacy were better than that of collagen peptide alone(P<0.01),obviously increased the activities of GSH-Px and SOD in serum,liver and heart,while reduced the concentrations of MDA(P<0.01),and inhibited the secretions of IL-1βand TNF-α(P<0.01),but had no significant effects on the concentrations of SCFAs in the colon though alterations in pH in colon were observed,compared with model group.Conclusion:The improvements of the loss of HYP and HA in aging skin in D-galactose induced aging mice in response to CPTP treatment might be due in part to an increase in the activities of antioxidant enzymes and a reduction in secretion of inflammatory cytokines.These findings may help to facilitate the application of CPTP in functional foods for beauty.

关 键 词：D-半乳糖衰老小鼠模型 羟脯氨酸 透明质酸 氧化压力 炎症因子 

分 类 号：TS201.4[轻工技术与工程—食品科学]