CRISPR/Cas生物传感器检测食源性病原体的研究进展  

Research Progress on CRISPR/Cas Biosensors for Detection of Foodborne Pathogens

作　　者：黄璐琦 孙旭飞 苑宁 范少华 张伟[1,4,5] Huang Luqi;Sun Xufei;Yuan Ning;Fan Shaohua;Zhang Wei(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,Hebei;College of Science and Technology,Hebei Agricultural University,Cangzhou 061100,Hebei;Hebei Software Vocational and Technical College,Baoding 071000,Hebei;College of Life Sciences,Hebei Agricultural University,Baoding 071000,Hebei;Hebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism,Baoding 071000,Hebei)

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]河北农业大学理工学院,河北沧州061100 [3]河北软件职业技术学院,河北保定071000 [4]河北农业大学生命科学学院,河北保定071000 [5]河北省人畜共患病原微生物分析与防控重点实验室,河北保定071000

出　　处：《中国食品学报》2022年第10期378-390,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金面上项目(32172288,31371772);河北省自然科学基金重点项目(C2019204342);河北省重点研究开发计划项目(18275501D);中央引导地方科技发展资金项目-基础研究项目(216Z5501G);河北省外专百人计划(360-0803-JSN-3YGS);河北省博士后科研项目(B2021005007)。

摘　　要：食源性病原体引起的食源性疾病严重威胁着人类的健康,灵敏且快速检测食源性病原体成为预防疾病发生的重要手段。生物传感器具有灵敏度高,无需富集即可实时定量,易于现场检测等优点。CRISPR/Cas系统与生物传感器结合可提高生物传感器检测的灵敏度和准确性。本文介绍CRISPR/Cas系统的分类及作用机制,回顾基于CRISPR/Cas的各种生物传感器检测食源性病原体的方法,包括荧光传感器、电化学传感器和侧流层析试纸,分析应用不同Cas蛋白检测的优缺点,为食源性病原体的检测提供借鉴。Foodborne diseases caused by foodborne pathogens seriously threaten human health. Sensitive and rapid detection of foodborne pathogens has become an important means to prevent disease occurrence. Biosensors have the advantages of high sensitivity, real-time quantification without enrichment, and easy on-site detection. The combination of CRISPR/Cas system combined with biosensors can improve the sensitivity and accuracy of biosensors detection. This article introduces the classification and mechanism of the CRISPR/Cas system, reviews various biosensors based on CRISPR/Cas for the detection of foodborne pathogens, including fluorescent sensors, electrochemical sensors, and lateral flow strip, and analyzes the advantages and disadvantages of different Cas protein detection, so as to provide reference for the detection of foodborne pathogens.

关 键 词：CRISPR/Cas 生物传感器 食源性病原体 

分 类 号：R155.5[医药卫生—营养与食品卫生学]