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作 者:李静[1] 李云清 林荔辉[1] 方平平[1] 祁建民[1] 陶爱芬[1] LI Jing;LI Yunqing;LIN Lihui;FANG Pingping;QI Jianmin;TAO Aifen(Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops/Fujian Provincial Key Laboratory of Crops by Design,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
机构地区:[1]福建农林大学作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室,福建福州350002
出 处:《亚热带农业研究》2022年第3期155-162,共8页Subtropical Agriculture Research
基 金:现代农业产业技术体系建设项目(CARS-19-E06);福建省南方麻类种质资源共享平台项目(PTJH20006);福建农林大学创新基金项目(KFA17164A,KFA19028A)。
摘 要:[目的]了解玫瑰茄花青素的研究现状,为其关键基因的挖掘和新种质的创制提供依据。[方法]从玫瑰茄花青素的组成成分、提取工艺、含量及品质的影响因素以及分子生物学研究等方面进行综合论述,并对今后的研究方向进行展望。[结果]目前,我国关于玫瑰茄花青素的研究主要侧重于应用方面,有关合成分子机制的研究相对薄弱。玫瑰茄花青素的提取方法比较多,其中乙醇提取法应用比较广,而酶提取法应用较少。品种基因型、非生物胁迫、采收时间及光照等均会影响玫瑰茄花青素的合成。玫瑰茄基因组尚未破译,花青素分子生物学研究相对落后。[结论]建议加强玫瑰茄种质资源的搜集、鉴定和利用研究,促进其新品种的选育;并深入分子生物学研究,通过分子标记辅助育种、基因编辑等技术创制新种质。[Purpose] Research progress of roselle anthocyanins was reviewed in this study to provide a theoretical basis for the screening of key genes for its synthesis and creation of new germplasm. [Method] This paper comprehensively reviewed the composition, extraction process, influencing factors of synthesis and molecular biology of roselle anthocyanins, and also discussed the future research direction. [Result] Current research of roselle anthocyanins mainly focuses on the application, while the molecular mechanism of its synthesis is barely investigated. Among the various extraction methods, extraction by ethanol is widely used, while extraction by enzyme is much less common. Factors like genotype, abiotic stress, harvest time and light affected the synthesis of roselle anthocyanins. As the roselle genome has not been deciphered, anthocyanin is still less understood from a molecular biological perspective. [Conclusion] Studies on the screening, identification and utilization of germplasm resources of roselle should be strengthened for the breeding of new varieties. Moreover, in-depth molecular biology study based on biotechnologies like molecular marker-assisted breeding and gene editing should be used to facilitate the creation of new germplasm.
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