双泵双柱UP-LC/MSMS法对烟熏肉制品中氯霉素、氟苯尼考和甲砜霉素的稳定性分析  

Stability analysis of chloramphenicol,florfenicol and thiamphenicol in smoked meat products by double-pump double-column UP-LC/MSMS

作　　者：曹俊杰 董榕贵 杨昌彪 于霞 马凯 黄永桥 简银池 肖雄枫 CAO Junjie;DONG Ronggui;YANG Changbiao;YU Xia;MA Kai;HUANG Yongqiao;JIAN Yinchi;XIAO Xiongfeng(Guizhou Testing Technology Research and Application Center,Guiyang 550014,China;Guizhou Academy of Testing and Analysis,Guiyang 550014,China)

机构地区：[1]贵州省检测技术研究应用中心,贵州贵阳550014 [2]贵州省分析测试研究院,贵州贵阳550014

出　　处：《贵州科学》2022年第5期77-81,共5页Guizhou Science

基　　金：贵州科学院青年基金(黔科院J字〔2021〕33号)。

摘　　要：建立了双泵双柱高效液相色谱/质谱法对烟熏肉制品中氯霉素、氟苯尼考和甲砜霉的快速分析方法。试样经氨化乙酸乙酯提取,浓缩后用(1+9)乙腈+水定容,正己烷去脂,得到样品待测液。试验结果:氯霉素类在0.5~10.0 ng/mL浓度范围内具有良好线性关系,相关系数均大于0.995;不同浓度加标回收率均在80.2%~109.2%之间,相对偏差小于14.6%:方法检出限为氯霉素0.1μg/kg,甲砜霉素和氟苯尼考0.5μg/kg。在30天周期性质稳定。此方法重现性好,灵敏度高,分析高效、快捷,能满足批量样品快速分析。The rapid determination method for the stability of chloramphenicol,florfenicol and thiamphenicol in smoked meat products by dual-pump dual-column UP-LC/MSMS was established.The sample was extracted by ammoniated ethyl acetate,concentrated,volume-stabilized by(1+9)acetonitrile and purified water,and degreased by n-hexane before testing.The results showed that the compounds had good linear relationship in the concentration range of 0.5 ng/mL to 10.0 ng/mL,the correlation coefficients were all greater than 0.995,the recoveries of different concentrations were between 80.2%and 109.2%,the relative standard deviations were less than 14.6%.The limit of detection was 0.1μg/kg for chloramphenicol,and 0.5μg/kg for thiamphenicol and florfenicol.In the 30-day cycle,the compounds were stable.The method has the advantages of good reproducibility,high sensitivity,and high efficiency,and can be used for the rapid analysis of large quantities of samples.

关 键 词：烟熏肉制品 氯霉素 氟苯尼考 甲砜霉素 双泵双柱 

分 类 号：TS207.3[轻工技术与工程—食品科学]