GSTP1基因遗传变异对结直肠癌术后接受辅助化疗患者复发及预后的影响  

Influence of genetic variation of GSTP1 gene on recurrence risk and prognosis of colorectal cancer patients receiving adjuvant chemotherapy after operation

作　　者：宣斌 王益民[1] XUAN Bin;WANG Yi-min(Department of General Surgery,First Hospital of Qinhuangdao City,Qinhuangdao Hebei 066000,China.)

机构地区：[1]秦皇岛市第一医院普外科,河北秦皇岛066000

出　　处：《临床和实验医学杂志》2022年第19期2072-2076,共5页Journal of Clinical and Experimental Medicine

基　　金：国家青年自然科学基金号(编号:81802372);秦皇岛市重点研发计划科技支撑项目(编号:202101A208)。

摘　　要：目的分析谷胱甘肽S-转移酶P-1(GSTP1)基因遗传变异对结直肠癌术后接受辅助化疗患者复发及预后的影响。方法回顾性选取2014年4月至2022年4月于秦皇岛市第一医院普外科行手术治疗的结直肠癌并同意行基因检测的133例患者资料,术后给予辅助化疗。化疗前采集患者的血液标本及组织标本进行基因检测,分析结直肠癌患者GSTP1基因突变特点及与患者临床特征的相关性。提取患者RNA,分析GSTP1 mRNA表达情况。采用Kaplan-Merier生存分析法对基因型与预后单变量分析,建立Cox风险比例模型实施校正。结果133例患者无进展生存期(DFS)为(4.67±0.52)年,总生存期(OS)为(6.32±0.43)年。经过基因多态性分析,结果显示GSTP1基因编码的I105V位点AA型98例,AG型32例,GG型3例,其与患者预后密切相关;随访患者复发及预后情况,显示AA基因型DFS、OS为(5.72±0.42)、(7.03±1.02)年,高于AG/GG基因型的(3.98±0.31)、(4.52±0.63)年,差异有统计学意义(P<0.05)。AG/GG基因型是患者OS的独立影响因素;I105V位点AG/GG基因型外周血中GSTP1 mRNA表达明显高于AA基因型,差异有统计学意义(P<0.05)。结论GSTP1基因I105V位点在GSTP1 mRNA表达中具有一定的介导作用,对结直肠癌患者术后辅助化疗效果及预后、复发风险有着直接的影响。Objective To analyze the effect of genetic variation of glutathione S-transferase P-1(GSTP1)gene on the recurrence risk and prognosis of colorectal cancer(CRC)patients receiving postoperative adjuvant chemotherapy.Methods The data of 133 patients with CRC who underwent surgical treatment at the Department of General Surgery,First Hospital of Qinhuangdao City from April 2014 to April 2022 and who agreed to undergo genetic testing were retrospectively selected,and adjuvant chemotherapy was given after surgery.Blood samples and tissue samples of patients were collected for gene detection,and the characteristics of GSTP1 gene mutation in CRC patients and their correlation with clinical characteristics of patients were analyzed.The patient RNA was extracted and the expression of GSTP1 mRNA was analyzed.Univariate analysis of genotype and prognosis was performed by Kaplan-Merier survival analysis,and a Cox proportional hazard model was established for correction.Results The progression-free survival(DFS)and overall survival(OS)of 133 patients were(4.67±0.52)years and(6.32±0.43)years,respectively;The I105V locus has 98 cases of AA type,32 cases of AG type and 3 cases of GG type,which are closely related to the prognosis of the patients.The recurrence and prognosis of the pa tients were followed up,and the DFS of the AA genotype was(5.72±0.42)years,and the OS was(7.03±1.02)years,which were higher than those of AG/GG genotype(3.98±0.31)and(4.52±0.63)years,the difference was statistically significant(P<0.05);AG/GG genotype was an independent influencing factor of OS;the expression of GSTP1 mRNA in peripheral blood of AG/GG genotype at I105V site was significantly higher than that of AA genotype,and the difference was statistically significant(P<0.05).Conclusion The I105V locus of GSTP1 gene has a certain mediating role in the expression of GSTP1 mRNA,and has a direct impact on the postoperative adjuvant chemotherapy effect,prognosis and recurrence risk of CRC patients.

关 键 词：结直肠癌 辅助化疗 GSTP1 基因遗传变异 复发 预后 

分 类 号：R735.34[医药卫生—肿瘤]