紫薇属植物叶绿体基因组研究进展  

Research Progress of Chloroplast Genome in Lagerstroemia spp.

作　　者：黄建睿 陈涛[2] 缪绅裕[1] HUANG Jianrui;CHEN Tao;MIAO Shenyu(School of Life Sciences,Guangzhou University,Guangzhou 510006,China;Fairy Lake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen 518004,China)

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]深圳市中国科学院仙湖植物园,广东深圳518004

出　　处：《广东农业科学》2022年第10期52-61,共10页Guangdong Agricultural Sciences

基　　金：广东省科技计划公益研究与能力建设专项(2018B030320007)。

摘　　要：紫薇属植物在全世界约有60种,已培育出500多个品种。紫薇属植物是优良的园林观赏植物,除花色艳丽夺目外,还有净化空气的特性,在园林和园艺应用中具有重要价值。目前,由于紫薇属植物频繁的种间杂交和基因渐渗,形态分类系统已不能满足紫薇属植物的遗传多样性研究。随着现代生物技术发展,基于叶绿体基因组的研究在物种亲缘关系研究中表现出明显优势,从叶绿体基因组中发掘的DNA片段及简单重复序列常用作植物分子标记,便于植物的鉴定与分类。迄今已完成22种紫薇属植物叶绿体基因组的全序列测定,利用贝叶斯法分析了22种紫薇属植物的系统发育关系,得出22种紫薇属植物为单系群;并归纳得出紫薇属叶绿体基因组大小约为150 kbp,最大的Lagerstroemia venusta长度为152 521 bp,最小的L.guilinensis长度为151 968 bp,其中LSC区长度为83~84 kbp,SSC区长度约16 kbp,IR区约25 kbp,叶绿体基因组中GC含量为37.6%~37.7%。综述了紫薇属植物叶绿体基因组的结构及其在DNA条形码、简单重复序列与系统发育中的应用,旨在了解紫薇属植物叶绿体全基因组的研究现状,提高对紫薇属在园林应用中的评价认识,为该属基于叶绿体基因组的种质资源鉴定、遗传和系统发育等方面深入研究奠定基础,为培育更多应用于园林观光的紫薇属植物提供数据支撑。There are about 60 species of Lagerstroemia in the world,and more than 500 species have been cultivated.Lagerstroemia species is an excellent garden ornamental plant.In addition to bright color and the characteristics of air purification,it has important value in garden and horticultural applications.At present,due to the frequent interspecific hybridization and gene introgression,the morphological classification system can not satisfy the genetic diversity study of Lagerstroemia.With the development of modern biotechnology,studies based on chloroplast genome have shown obvious advantages in the study of species relationship.DNA fragments and simple repeat sequences extracted from chloroplast genomes are often used as molecular markers for identification and classification of plants.So far,the chloroplast genomes of 22 species of Lagerstroemia have been sequenced.The phylogenetic relationships of 22 species of Lagerstroemia have been analyzed by Bayes’ method,and 22 species of Lagerstroemia are monophyletic groups.It is concluded that the chloroplast genome size is about 150 kbp,the largest is L.venusta with a length of 152 521 bp,and the smallest was L.guilinensis with a length of 151 968 bp.The length of LSC region is 83-84 kbp,the length of SSC region is 16 kbp,the length of IR region is 25kbp,and the content of GC in chloroplast genome is 37.6%-37.7%.The research reviews the structure of chloroplast genome of Lagerstroemia and its application in DNA barcoding,SSR and phylogeny in order to understand the research status of the chloroplast genomes of Lagerstroemia and improve the evaluation and understanding of the application of Lagerstroemia in landscape architecture.It will lay a foundation for further research on germplasm resources identification,genetics and phylogeny of Lagerstroemia based on chloroplast genome and provide data support for breeding more Lagerstroemia plants used in garden sightseeing.

关 键 词：紫薇属 叶绿体基因组 系统发育 DNA条形码 简单重复序列 

分 类 号：S685.99[农业科学—观赏园艺]