大孔强酸树脂膨胀床吸附提取ε-聚赖氨酸的研究  

Extraction ofε-poly-L-lysine from fermentation broth by expanded bed adsorption using macroporous strong acid resin as adsorbent

作　　者：刘洋 王靓[1,2] 张宏建[1,2] 陈旭升 张建华[1,2] LIU Yang;WANG Liang;ZHANG Hongjian;CHEN Xusheng;ZHANG Jianhua(School of Biotechnology,Jiangnan University,Wuxi 214122,China;The Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]工业生物技术教育部重点实验室(江南大学),江苏无锡214122

出　　处：《食品与发酵工业》2022年第22期9-17,共9页Food and Fermentation Industries

基　　金：江苏省自然科学基金项目(BK20191332);国家自然科学基金项目(31671846)。

摘　　要：为了取消ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)提取工艺中的固液分离操作,降低分离提取成本和提高提取效率,该研究建立了一种利用膨胀床直接从发酵液中吸附提取ε-PL的新工艺。首先通过静态吸附进行大孔强酸树脂筛选,再采用两种等温线模型研究293~310 K下树脂吸附ε-PL热力学行为,再对膨胀床吸附提取ε-PL的工艺参数进行优化,最后构建并优化了三柱串联膨胀床吸附提取ε-PL循环工艺条件。结果表明,大孔强酸钠型树脂SQD-04适用于膨胀床工艺,且其吸附ε-PL符合Langmuir吸附等温线模型,热力学参数分析发现SQD-04树脂吸附ε-PL过程为放热反应;在三柱串联膨胀床循环工艺中,初始进料质量浓度25 g/L,树脂初始高径比2.7,膨胀比1.6,v_(1)=1 BV/h时进料,13个柱体积时3#柱穿透,1#柱的树脂饱和度可达0.76,总收率为98.20%。研究结果为利用膨胀床直接从发酵液中吸附提取ε-PL工艺的工业应用提供了重要指导,也为升级换代现有ε-PL提取工艺提供了新思路。In order to cancel the solid-liquid separation operation in the extraction process of ε-poly-L-lysine(ε-PL), reduce the cost of separation and extraction and improve the extraction efficiency, this study establishes a new process for directly adsorbing and extracting ε-PL from the fermentation broth using expanded bed absorption. Firstly, static adsorption experiments compared and screened the adsorp-tion and desorption parameters of eight macroporous strong acid resins. Secondly, the Langmuir adsorption isotherm model and the Freun-dlich adsorption isotherm model were used to linearly fit the experimental results of the resin adsorption of ε-PL in fermentation broth at 293-310 K, and the experiment studied thermodynamic parameters. Thirdly, single-factor experiments optimized the process parameters(expansion ratio, initial height-diameter ratio of resin, initial feed mass concentration) of ε-PL in the fermentation broth. Finally, the experiments constructed a three-column series expanded bed cycle process and optimized its feed rate. The results showed that the macroporous strong acid resins SQD-04, D072 and D061 were screened out according to the experimental results of the resin working exchange capacity, desorption rate, protein removal rate and pigment removal rate, using HPLC to further analyze the purity and the degree of polymerization of the eluents of the three resins, the SQD-04 resin was screened out to be more suitable for the expanded bed absorption. The correlation coefficient of Langmuir adsorption isotherm model linearly fitting the experimental results of Naform SQD-04 resin adsorption of ε-PL in fermentation broth was greater than 0.99. Thermodynamic parameters showed that the adsorption process was an exothermic re-action and the total disorder of the system increases and adsorption of ε-PL in fermentation broth by Naform SQD-04 resin was more suit-able at 293 K. In the single factor optimization of expanded bed process parameters, if the column efficiency is considered, the initial fee

关 键 词：Ε-聚赖氨酸 膨胀床 离子交换 吸附等温线模型 热力学 

分 类 号：TQ317[化学工程—高聚物工业]