凉粉草抑制α-葡萄糖苷酶活性成分快筛法的初步研究  

Preliminary Study on Fast Screening Method ofα-Glucosidase Active Component from Mesona Chinensis Benth

作　　者：冯白茹 莫颖华 Feng Bairu;Mo Yinghua(Huizhou Health Sciences Polytechnic,Huizhou 516000,China)

机构地区：[1]惠州卫生职业技术学院,广东惠州516025

出　　处：《山东化工》2022年第20期123-126,共4页Shandong Chemical Industry

摘　　要：目的:研究从凉粉草中快速发现具有α-葡萄糖苷酶抑制活性的化合物。方法:利用亲和超滤技术从凉粉草中快速发现具有α-葡萄糖苷酶抑制活性化合物,并利用紫外可见分光光度法测定其α-葡萄糖苷酶抑制活性。结果:凉粉草提取物对α-葡萄糖苷酶的抑制活性为0.73%~19.72%,不同产地的凉粉草的提取物对α-葡萄糖苷酶的抑制活性各有差异;选用95%乙醇作为提取溶剂时,凉粉草提取物对α-葡萄糖苷酶的抑制活性最高。结论:本快筛法操作简单、易行,可快速从凉粉草中筛选出具有α-葡萄糖苷酶抑制活性的成分。Objective:To study the rapid discovery ofα-glucosidase inhibitory compounds from mesona chinensis benth.Methods:The compounds withα-glucosidase inhibitory activity were rapidly identified by affinity ultrafiltration technology,and theα-glucosidase inhibitory activity was determined by UV-VIS spectrophotometry.Results:The activity ofα-glucosidase was 0.73%~19.72%.The activity ofα-glucosidase was different in the extracts from different regions.When 95%ethanol was used as the extraction solvent,theα-glucosidase inhibitory activity of the extract was the highest.Conclusion:This fast screening method is simple and feasible,and can be used to screenα-glucosidase inhibitory constituents from mesona chinensis Benth.

关 键 词：凉粉草 Α-葡萄糖苷酶 亲和超滤 

分 类 号：TQ460[化学工程—制药化工]