雪腐镰刀菌和酱油曲霉转化橙皮苷生成橙皮素单葡萄糖苷效果  

Effects of Fusarium nivale and Aspergillus sojae on Transformation of Hesperidin into Hesperitin-7-O-glucoside

作　　者：张风亭 胡坦[1] 潘思轶[1] ZHANG Fengting;HU Tan;PAN Siyi(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学与技术学院,湖北武汉430070

出　　处：《食品科学》2022年第22期145-150,共6页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-26-07B);中央高校基本科研业务费专项(2662019PY016,2662020SPPY011);湖北省技术创新专项重大项目(2018ABA072)。

摘　　要：为比较单一菌种与混合菌种在30℃、160 r/min条件下转化橙皮苷生成橙皮素单葡萄糖苷的效果,以橙皮苷为原料,雪腐镰刀菌和酱油曲霉为生产菌种进行液体深层发酵,采用高效液相色谱法和超高效液相色谱-质谱联用仪对单一菌种(雪腐镰刀菌、酱油曲霉)和混合菌种(雪腐镰刀菌-酱油曲霉1∶1)不同时间段发酵液中发酵产物、α-L-鼠李糖苷酶活力以及发酵液pH值进行分析。结果表明:混合菌种与单一菌种转化橙皮苷生成橙皮素单葡萄糖苷差异显著(P<0.05),混合菌种发酵产生最大酶活力(104.81 U/mL)明显高于单一菌种(24.73、26.17 U/mL),二者发酵均在酸性条件(pH 4~6)下完成。混合菌种较优的发酵效果可为综合开发橙皮提供又一途径,充分利用柑橘加工副产物的同时避免了环境污染等问题的发生。In order to compare the conversion efficiencies of hesperidin into hesperitin-7-O-glucoside by single-and mixed-strain at 30℃and 160 r/min,hesperidin was transformed by submerged fermentation using single and mixed(1:1)cultures of Fusarium nivale and Aspergillus sojae,and the fermentation products and theα-L-rhamnosidase activity of the fermentation broth the at different time points during fermentation were determined by high performance liquid chromatography(HPLC)and ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS)and the pH of the fermentation broth was measured as well.The results showed that there was a significant difference between the mixed-and single-strain fermentations in the transformation of hesperidin to hesperitin-7-O-glucoside(P<0.05).The mixed-strain fermentation gave a higherα-L-rhamnosidase activity(104.81 U/mL)than the single-strain fermentations(24.73 and 26.17 U/mL),and the fermentation of both strains was completed under acidic condition(pH 4-6).Mixed-strain fermentation could provide another way for the comprehensive development of orange peel,which could make full use of citrus by-products and at the same time avoid environmental pollution.

关 键 词：橙皮苷 发酵 橙皮素单葡萄糖苷 高效液相色谱法 超高效液相色谱-质谱 

分 类 号：Q815[生物学—生物工程]