鸡[土从]菌基因组测序和比较基因组分析  

Genome Sequencing and Comparative Genomic Analysis of Termitornyces albuminosus

作　　者：黎勇[1,2] 孙明伟 杜婉婷 邓佳 陈慧 史典义 LI Yong;SUN Mingwei;DU Wanting;DENG Jia;CHEN Hui;SHI Dianyi(Department of Life Sciences,Neijiang Normal University,Neijiang 641100,Sichuan,China;Sichuan Science&Technology Resources Sharing Service Platform of Special Agricultural Resources in Tuojiang River Basin,Neijiang 641100,Sichuan,China)

机构地区：[1]内江师范学院生命科学学院,四川内江641100 [2]沱江流域特色农业资源四川省科技资源共享服务平台,四川内江641100

出　　处：《食用菌学报》2022年第6期1-13,共13页Acta Edulis Fungi

基　　金：内江师范学院大学生创新创业训练计划(X2021089)。

摘　　要：对采集自四川省内江市东兴区高桥村灌木林的鸡[土从]菌(Termitomyces albuminosus)进行基因组测序、组装、基因功能注释、碳水化合物酶(carbohydrate-active enzyme,CAZy)预测、进化树构建、同源序列比对及简单重复序列(simple sequence repeat,SSR)分子标记开发和PCR扩增验证的研究工作。结果表明:鸡[土从]菌全基因组共存在1.71×10^(3)个蛋白质编码基因,平均长度为1.65×10^(3) bp;CAZy功能注释聚类分析表明,黑腹果蝇(Drosophila melanogaster)、内华达古白蚁(Zootermopsis nevadensis)、面包酵母(Saccharomyces cerevisiae)和裂体吸虫(Schistosoma mansoni)聚类到同一大组,鸡[土从]菌与其他物种聚类到另一大组,且与刺芹侧耳(Pleurotus eryngii)和糙皮侧耳(P.ostreatus)聚类到更小分组;进化树分析表明,蚁巢伞属真菌与常见的栽培食用菌之间存在一定的进化距离,与金针菇(Flammulina velutipes)、猴头菌(Hericium erinaceus)和香菇(Lentinula edodes)的进化距离较近;采用SSR标记对收集的鸡[土从]菌进行分析,发现一地域分布的鸡[土从]菌在遗传上存在较高的相似性。A Termitomyces albuminosus sample collected from a shrubbery in Gaoqiao village,Dongxing District,Neijiang City,Sichuan Province was sequenced for whole genome and the assembled genome was subjected to gene function annotation,carbohydrate-active enzyme(CAZy)prediction,phylogenetic analysis,homologous sequence alignment,simple sequence repeat(SSR)molecular marker development and PCR amplification verification.The results showed that a total of 1.71×10^(3) protein-coding genes were identified in the whole genome with an average length of 1.65×10^(3) bp.The cluster analysis of CAZy functional annotation showed that Drosophila melanogaster,Zootermopsis nevadensis,Saccharomyces cerevisiae and Schistosoma mansoni were clustered in the same big group,whereas T.albuminosus was clustered with Pleurotus eryngii and P.ostreatus in an individual branch of another big group.The phylogenetic analysis based on full-length 18 S rRNA gene sequences showed that there was a certain distance between the genus Termitomyces and common cultivated edible mushroom species,and among them Flammulina velutipes,Hericium erinaceus and Lentinula edodes were relatively closer to Termitomyces spp.compared with other species.Amplification of SSR markers indicated that Termitomyces spp.distributed in a region were highly similar in heredity.

关 键 词：鸡[土从]菌 白蚁 基因组组装 比较基因组 简单重复序列 

分 类 号：S646[农业科学—蔬菜学]