基于顶空气相色谱质谱联用技术的紫雪散挥发性成分研究  

Study on volatile components in Zixue Powder by headspace gas chromatography mass spectrometry

作　　者：宋玲玲 穆琦瑄 王帆[4] 张慧[4] 柴欣[1,2,3] 王跃飞[1,2,3] 崔英 SONG Lingling;MU Qixuan;WANG Fan;ZHANG Hui;CHAI Xin;WANG Yuefei;CUI Ying(Institute of Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Medicine,Tianjin 301617,China;Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis,Tianjin 301617,China;Tianjin Hongrentang Pharmaceutical Co.,Ltd.,Tianjin 300385,China)

机构地区：[1]天津中医药大学中医药研究院,天津301617 [2]天津中医药大学组分中药国家重点实验室,天津301617 [3]天津市中药化学与分析重点实验室,天津301617 [4]天津宏仁堂药业有限公司,天津300385

出　　处：《天津中医药》2022年第11期1470-1475,共6页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市科技计划项目(20ZYJDJC00070)。

摘　　要：[目的]建立顶空气相色谱质谱联用技术(HS GC-MS)快速鉴定紫雪散中挥发性成分的方法,分析不同批次、不同工艺的紫雪散样品中挥发性成分的差异。[方法]采用HS GC-MS技术建立紫雪散中挥发性成分的分析方法,利用NIST 17标准谱库鉴定紫雪散挥发性成分,并归属各成分的药材来源,运用峰面积归一化法测定各成分的相对含量。[结果]从紫雪散中共鉴定了46个挥发性成分,包括醇类、酯类、醛类、酮类、酸类、烃类及含氮化合物等,其中最主要成分为麝香酮。不同批次、不同工艺的紫雪散样品中各成分相对含量存在较大差异。[结论]系统阐释了紫雪散中挥发性成分,为科学评价及有效控制紫雪散质量提供了依据。[Objective]To establish a rapid analytical method for identifying the volatile components in Zixue Powder by headspace gas chromatography mass spectrometry(HS GC-MS)and evaluate the difference of the volatile components among different batches and different processes of Zixue Powder sample.[Methods]The analytical method of volatile components in Zixue Powder was established by HS GC-MS.The compounds were identified by NIST 17 database retrieval and traced to the herbal sources.The relative content of each compound was determined by peak area normalization method.[Results]The 46 volatile compounds were characterized from Zixue Powder,including alcohols,esters,aldehydes,ketones,acids,hydrocarbons and nitrogenous compounds,and the main compound was muscone.There were great differences in the relative content of the volatile components among different batches and processes of Zixue Powder sample.[Conclusion]The volatile components in Zixue Powder were characterized,which provides a basis for scientific evaluation and effective quality control of Zixue Powder.

关 键 词：紫雪散 挥发性成分 顶空进样 气质联用 

分 类 号：R284.1[医药卫生—中药学]