超声辅助提取箬竹叶多糖工艺及抗氧化活性研究  

Response surface methodology for optimization of ultrasonic-assisted extraction of polysaccharide from Indocalamus leaves and its antioxidation

作　　者：周路坦[1] 温君 李盈盈 李秀红[2] ZHOU Lutan;WEN Jun;LI Yingying;LI Xiuhong(Zhengzhou Railway Vocational and Technical College,Zhengzhou 450000;The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000)

机构地区：[1]郑州铁路职业技术学院,郑州450000 [2]郑州大学第一附属医院,郑州450000

出　　处：《中国食品添加剂》2022年第11期69-76,共8页China Food Additives

摘　　要：采用单因素及响应面优化超声辅助法提取箬竹叶多糖工艺,并对小鼠进行28天灌胃不同剂量箬竹叶多糖后测定肝脏和血清中总抗氧化能力、超氧化物歧化酶含量、谷胱甘肽过氧化物酶含量和丙二醛含量以研究其体内抗氧化活性和以DPPH自由基清除率研究其体外抗氧化能力。结果表明:箬竹叶多糖的最佳提取条件为:料液比1/20(g/mL)、超声时间40min、超声功率400W、提取温度75℃,箬竹叶多糖提取率为5.59mg/g。箬竹叶多糖有较强的体内和体外抗氧化活性,其可以显著提高肝脏和血清中总抗氧化能力、超氧化物歧化酶含量、谷胱甘肽过氧化物酶含量,降低丙二醛含量,对清除DPPH自由基可高达93.25%。箬竹叶多糖可作为一种天然食品添加剂。Ultrasonic-assisted extraction process of polysaccharide from Indocalamus leaves was optimized by single factor and response surface methodology. After intragastric administration of polysaccharide from Indocalamus leaves at different doses for 28 days,the total antioxidant capacity,superoxide dismutase content,glutathione peroxidase content and malondialdehyde content in liver and serum were measured. The antioxidant activity of DPPH was studied in vitro.The results showed that the optimum extraction conditions were as follows :material-to-water ratio 1/20(g/mL), ultrasonic time 40min,ultrasonic power 400W,extraction temperature 75℃. Under the above conditions,the yield of polysaccharide from Indocalamus leaves was 5.59mg/g. Polysaccharides had a strong antioxidant activity in vivo and in vitro,the activity of superoxide dismutase and glutathione peroxidase from liver and serum were higher than the control,and the content of malondialdehyde in liver and serum had a remarkable reduction effect,93.25% of DPPH free radicals was scavenged. Polysaccharide from Indocalamus leaves could be used as a natural food additive.

关 键 词：箬竹叶 多糖 超声辅助提取 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学] O644.3[轻工技术与工程—食品科学与工程] O629.12[理学—物理化学]