EMR-Lipid固相萃取结合液相色谱串联质谱法快速测定鸡蛋中金刚烷胺和金刚乙胺残留量  

Rapid determination of amantadine and rimantadine residues in egg by EMR-Lipid solid phase extraction combined with liquid chromato-graphy-tandem mass spectrometry

作　　者：姜英杰[1] 葛子欣 翟玮玮[1] 苏晶 JIANG Yingjie;GE Zixin;ZHAI Weiwei;SU Jing(Jiangsu Food and Pharmaceutical Science College,Huai’an 223003;Huai’an Food and Drug Inspection Institute,Huai’an 223003)

机构地区：[1]江苏食品药品职业技术学院,淮安223003 [2]淮安市食品药品检验所,淮安223003

出　　处：《中国食品添加剂》2022年第11期218-224,共7页China Food Additives

摘　　要：采用EMR-Lipid增强型脂质去除固相萃取结合高效液相色谱串联质谱建立了快速测定鸡蛋中金刚烷胺和金刚乙胺的方法。样品由2%甲酸乙腈提取后,经EMR-Lipid固相萃取柱净化,以乙腈-0.1%甲酸水为流动相,采用T3色谱柱(100mm×2.1mm,1.8μm)进行分离,正离子模式多反应监测条件下,采用基质配标外标法进行定量。在此条件下,金刚烷胺和金刚乙胺在1.0~20.0μg/kg范围内,线性关系良好(r≥0.998),方法的检出限为0.5μg/kg,定量限1.0μg/kg,平均回收率为76.5%~106.8%,相对标准偏差为2.41%~6.81%。该方法样品前处理快速简便,减少了同位素内标的使用,节约成本,适用于大批量样本的快速检测。A rapid method by EMR-Lipid solid phase extraction combined with liquid chromatography–tandem mass spectrometry was established for determining amantadine and rimantadine residues in eggs. The sample was extracted with acetonitrile with 2% formic acid,purified by EMR-Lipid solid phase extraction column,separated by T3 chromatographic column(100mm×2.1mm,1.8μm)with acetonitrile-water 0.1% formic acid as mobile phase. The analysis were performed by electrospray positive ion scanning mode under multiple reaction monitoring with matrix matched standard method for quantification. The method had good linear relationship(R > 0.998)in the range of 1.0~20.0μg/kg for amantadine and rimantadine residues in eggs,the limits of detection were 0.5 μg/kg,the lower limits of quantification were 1.0 μg/kg,the average recoveries were 76.5%~106.8%,and the relative standard deviations were 2.41%~6.81%. This method is simple and fast. In addition,since it does not use isotopic internal standard,cost is reduced. This method is suitable for a rapid determination of large samples.

关 键 词：EMR-Lipid固相萃取 高效液相色谱串联质谱法 金刚烷胺 金刚乙胺 鸡蛋 

分 类 号：TS207.3[轻工技术与工程—食品科学]