负载纳米羟磷灰石的结冷胶修复大鼠下颌骨缺损的效果评价  

Repair of mandibular defects with hydrogel loaded with nano-hydroxyapatite in rats

作　　者：许来俊 袁鹤[1] 叶青 李继遥[1] XU Lai-jun;YUAN He;YE Qing;LI Ji-yao(,State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases,Department of Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,Sichuan Province;Department of Endodontics,Xiangya School of Stomatology&Xiangya Stomatological Hospital,Central South University,Changsha 410008,Hunan Province,China)

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院牙体牙髓病科,四川成都610041 [2]中南大学湘雅口腔医学院(湘雅口腔医院)牙体牙髓病科,湖南长沙410008

出　　处：《上海口腔医学》2022年第5期449-453,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81701028);湖南省自然科学基金(2022JJ40733);湖南省卫生健康委科研计划项目(202208015033)。

摘　　要：目的:观察负载纳米羟磷灰石的结冷胶(GG/nHA)修复大鼠下颌骨缺损的效果。方法:于16只SD大鼠下颌骨制备直径为5 mm的临界骨缺损,随机分为2组,实验组骨缺损区注入GG/nHA,对照组骨缺损区填充可吸收性明胶海绵。术后4周、8周处死大鼠,以Micro-CT定量分析骨组织愈合情况。苏木精-伊红(H-E)染色和马松(Masson)染色定性评估骨组织修复情况。采用GraphPad Prism 8.0软件包对数据进行统计学分析。结果:制备的GG/nHA具有良好的可注射性,可经注射器推出至骨缺损区。术后4周和8周,实验组缺损区的骨生成量及骨体积分数(BV/TV)均显著高于对照组(P<0.05)。H-E染色和Masson染色均见实验组较对照组有更多新骨形成。结论:GG/nHA可以注射形式注入下颌骨缺损区,促进其愈合,有望成为微创修复口腔颌面部骨缺损的新型材料。PURPOSE:To observe the effect of gellan gum loaded with nano-hydroxyapatite(GG/nHA)on repairing mandibular defect in rats.METHODS:Critical bone defects with 5 mm in diameter on the mandible of 16 SD rats were created and randomly divided into two groups.The bone defects in the experimental group were injected with GG/nHA and the control group were filled with absorbable gelatin sponge.The rats were sacrificed at 4 and 8 weeks after operation.The bone tissue healing was evaluated by Micro-CT.Bone tissue repairing effect was evaluated by hematoxylin-eosin(H-E)staining and Masson staining.GraphPad Prism 8.0 software package was used for statistical analysis.RESULTS:The prepared GG/nHA had a good injectability and could be delivered to the bone defect area with a syringe.Four and 8weeks after operation,the newly formed bone and bone volume fraction(BV/TV)in the experimental group were higher than those in the control group,and the difference was statistically significant(P<0.05).Larger number of new bone were observed in the experimental group than the control group by H-E staining and Masson staining.CONCLUSIONS:GG/n HA can be injected into the mandibular defect area to promote its healing,and it is expected to be used as a novel biomaterial for minimally invasive repair of oral and maxillofacial bone defects.

关 键 词：颌骨缺损 水凝胶 结冷胶 纳米羟磷灰石 骨再生 

分 类 号：R783.1[医药卫生—口腔医学]