菠萝蜜多糖对小鼠肠道菌群的调节作用  

Modulatory Effect of Polysaccharide from Artocarpus heterophyllus Lam.(Jackfruit)Pulp on Gut Microbiota in Mice

作　　者：陈玉子 谭乐和[2] 刘启兵 吴刚[2,4] 张彦军 徐飞[2,4] 朱科学 CHEN Yuzi;TAN Lehe;LIU Qibing;WU Gang;ZHANG Yanjun;XU Fei;ZHU Kexue(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning 571533,China;Department of Pharmacology,School of Basic Medicine and Life Sciences,Hainan Medical College,Haikou 571199,China;Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province,Wanning 571533,China)

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]中国热带农业科学院香料饮料研究所,海南万宁571533 [3]海南医学院基础医学与生命科学学院药理学教研室,海南海口571199 [4]海南省特色热带作物适宜性加工与品质控制重点实验室,海南万宁571533

出　　处：《食品工业科技》2022年第24期189-196,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金项目(31901649);海南省重点研发计划项目(ZDYF2020218);中国热带农业科学院基本科研业务费专项资金(1630142022009)。

摘　　要：为探究菠萝蜜多糖(JFP-Ps)对小鼠肠道菌群多样性及其结构的影响,将24只雄性昆明小鼠随机分为四组:对照组、JFP-Ps(50 mg/kg BW)组、JFP-Ps(100 mg/kg BW)组和JFP-Ps(200 mg/kg BW)组,连续灌喂2周后,收集新鲜小鼠粪便,提取粪便DNA扩增肠道菌群16S rRNA基因的V3~V4区,通过Illumina高通量测序技术研究菠萝蜜多糖对小鼠肠道菌群的调节作用。结果表明,JFP-Ps可通过调节拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)等肠道优势菌群的组成和结构,增加肠道菌群的多样性。通过属水平分析发现,JFP-Ps可增加拟杆菌属(Bacteroides)、异杆菌属(Allobaculum)、乳酸杆菌属(Lactobacillus)和副拟杆菌(Parabacteroides)等产SCFAs菌属的丰度,减少普雷沃菌属(Prevotella)等菌属丰度,发挥肠道微生物调节作用,改善宿主肠道微生态健康,研究结果为JFP-Ps在肠道益生产品的研发应用奠定理论基础。The present study aimed to investigate the modulatory effect of polysaccharide from Artocarpus heterophyllus Lam.pulp(JFP-Ps)on the diversity and structure of gut microbiota in mice.24 male Kunming mice were randomly divided into four groups:Control group,JFP-Ps(50 mg/kg BW)group,JFP-Ps(100 mg/kg BW)group and JFP-Ps(200 mg/kg BW)group.The colonic feces of mice were collected after treating with JFP-Ps in different doses for 2 weeks.Then the DNA was extracted and the V3~V4 region of the 16S rRNA gene of the intestinal flora was amplified.The regulatory effect of JFP-Ps on the gut microbiota of mice was studied using Illumina Miseq high-throughput sequencing technology.The results showed that Bacteroidetes and Firmicutes were the dominant intestinal flora,and JFP-Ps modulated the composition of Bacteroidetes and Firmicutes in comparison with the control group.At the genus level,the abundance of SCFAs-producing bacteria,including Bacteroides,Allobaculum,Lactobacillus and Parabacteroides were significantly increased in response to JFP-Ps treatment,but a significant decrease in Prevotella.These results indicated that JFP-Ps could improve the diversity of gut microbiota by regulating the composition and structure of dominant bacteria such as Bacteroidetes and Firmicutes.This study would provide a theoretical basis for JFP-Ps in intestinal prebiotics.

关 键 词：菠萝蜜多糖 肠道菌群 调节作用 高通量测序 菌群多样性 

分 类 号：TS201.3[轻工技术与工程—食品科学]