蔗果三糖及低聚果糖对T细胞分泌干扰素γ的调节作用  

Regulatory effects of kestose and fructooligosaccharides on interferon-γ secretion of T cell

作　　者：郝占西 徐笑非 吴少辉 魏远安 HAO Zhan-xi;XU Xiao-fei;WU Shao-hui;WEI Yuan-an(Quantum Hi-Tech(Guangdong)Biological Co.,Ltd,Jiangmen,Guangdong 529081,China;不详)

机构地区：[1]量子高科(广东)生物有限公司,广东江门529081

出　　处：《中国微生态学杂志》2022年第9期1002-1005,共4页Chinese Journal of Microecology

基　　金：广东省防控新型冠状病毒感染科技攻关应急专项(2020A111129035);江门市高新区创新拔尖人才专项(2019ITP01)。

摘　　要：目的研究蔗果三糖及低聚果糖(fructooligosaccharides,FOS)对T细胞分泌干扰素(interferon-,IFN-γ^(+))的调节作用。方法从外周血单个核细胞分离培养T细胞。在培养基中添加5mg/mL的蔗果三糖或FOS并检测T细胞的诱导分化。平行设置空白对照。经过3d的原代培养或6d的原代及传代培养,采用流式细胞术检测IFN-γ^(+)T细胞的比例,酶联免疫吸附法测定细胞培养液的IFN-γ^(+)浓度。结果经过3d的原代培养,蔗果三糖组IFN-γ^(+)T细胞比例(47.8100%±4.8800%)显著高于FOS组(36.9600%±4.5800%,P=0.0002)或空白对照组(31.4000%±3.8900%,P=0.0001),蔗果三糖组细胞培养液的IFN-γ^(+)浓度(848.5100 ng/mL±269.0000ng/mL)也显著高于FOS组(357.8700ng/mL±87.3000ng/mL,P=0.0040)或空白对照组(283.5100ng/mL±76.0300ng/mL,P=0.0001);而且,经过6d的原代及传代培养,无论传代之后是否添加蔗果三糖与FOS,蔗果三糖组IFN-γ^(+)T细胞比例(32.0600%±5.2600%;31.7900%±4.8600%)始终显著高于FOS组(22.1300%±4.5400%,P=0.0078;21.8900%±4.4800%,P=0.0086)或空白对照组(18.6500%±2.6900%,P=0.0071;17.8300%±3.1300%,P=0.0074)。结论蔗果三糖有助于T细胞分化并促进T细胞分泌IFN-γ^(+)。ObjectiveTo explore the regulatory effects of kestose and fructooligosaccharides(FOS)on interferon-(IFN-)secretion of T cell.Methods T cell isolated from peripheral blood mononuclear cells was cultured and proliferated.5 mg/mL kestose or FOS were added to the culture media of T cell to test the induced differentiation.A blank control was set in parallel test.After 3 days of primary culture or 6 days of primary culture and subculture,the percentage of IFN-γ^(+)T cell was assessed by using flow cytometry and the concentration of IFN-in the culture media was determined with enzyme linked immunosorbent assay.ResultsAfter 3 days of primary culture,the percentage of IFN-γ^(+)T cell in kestose group(47.8100%±4.8800%)was significantly higher than that in FOS group(36.9600%±4.5800%,P=0.0002)or blank control(31.4000%±3.8900%,P=0.0001).Meanwhile,the concentration of IFN-in the culture media from kestose group(848.5100 ng/mL±269.0000 ng/mL)was also significantly higher than that from FOS group(357.8700 ng/mL±87.3000 ng/mL,P=0.0040)or blank control(283.5100 ng/mL±76.0300 ng/mL,P=0.0001).And,the percentage of IFN-γ^(+)T cell in kestose group(32.0600%±5.2600%;31.7900%±4.8600%)was always higher than that in FOS group(22.1300%±4.5400%,P=0.0078;21.8900%±4.4800%,P=0.0086)or blank control(18.6500%±2.6900%,P=0.0071;17.8300%±3.1300%,P=0.0074)after 6 days of primary culture and subculture,with or without kestose and FOS in the culture media after cell passage.Conclusion Kestose helps T cell to differentiate and promotes the secretion of IFN-γ from T cell.

关 键 词：蔗果三糖 T细胞 干扰素Γ 低聚果糖 外周血单个核细胞 

分 类 号：R151.3[医药卫生—营养与食品卫生学]