无二甲基亚砜细胞冻存技术的研究进展及展望  

Research progress and the prospect of cell cryopreservation of dimethyl sulfoxide-free

作　　者：贺晨宇 姜可伟[1,2] 王晨一 王杉[1,2] He Chenyu;Jiang Kewei;Wang Chenyi;Wang Shan(Department of Gastroenterological Surgery,Peking University People’s Hospital,Beijing 100442,China;Laboratory of Surgical Oncology,Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research,Peking University People’s Hospital,Beijing 100442,China)

机构地区：[1]北京大学人民医院胃肠外科,100442 [2]北京大学人民医院外科肿瘤研究室结直肠癌诊疗研究北京市重点实验室,100442

出　　处：《中华实验外科杂志》2022年第10期2049-2052,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金委科学中心项目(62088101)。

摘　　要：冷冻保存技术已成为细胞治疗、辅助生殖、组织工程和疫苗储存等生物医学应用的必要方法。冰晶的形成是冷冻保存的主要限制因素,并会对生物样本造成致命性损伤。二甲基亚砜(DMSO)是传统上常用的冷冻保护剂,然而考虑到DMSO自身细胞毒性和给药后对机体产生的潜在不良反应,因此寻找具有安全性和有效性的替代DMSO方法的需求逐渐增长。本文拟概述多种治疗性细胞的无DMSO保存的研究进展及面临的问题和挑战,以期推动新型细胞冷冻保存技术更好地开展。Cryopreservation has become an essential part of biomedical applications,such as cell-based therapy,assisted reproductive technology,tissue engineering and vaccine storage.Ice crystal formation is a major limiting factor in cryopreservation,which can cause fatal damage to biological samples.Dimethyl sulfoxide(DMSO)is commonly used as a cryoprotectant.However,considering the cytotoxicity of DMSO and its potential side effects after administration,the need to find a safe and effective alternative to DMSO is increasing.This review summarizes the research progress and challenges of DMSO-free preservation of a variety of therapeutic cells,in order to promote the development of new cell cryopreservation technology.

关 键 词：冷冻保存 无二甲基亚砜 海藻糖 抗冻蛋白 

分 类 号：R-331[医药卫生]